Abstract:Inter-simple sequence repeat（ISSR）was used to determine the genets of Duchesnea indica. The results show that 6 ISSR primers amplified 62 DNA bands in 22 ramets. Each of the primers amplified 4~16 bands, the average amplified band was 10.3. The use of six primer pairs 22 ramets cloned amplified DNA bands have 28 kinds of type, which has nine specific bands. Comprehensive analysis of these bands, ascertaining that 22 ramets belong to 16 genets. According to the banding patterns, all ramets tested in this study were distinguished from each other by four of 6 selected primers. It was shown that the ISSR technique is a reliable and effective tool to identify the genets of D.indica. At the same time, the polymorphic proportion of the bands amplified by each primer, with an average of 90.3%.