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作 者 ：余乃通;孙玉娟;张雨良;罗志文;刘志昕*

期 刊 ：植物研究 2012年 32卷 1期 页码：47-53

关键词：香蕉；巴西橡胶；cat基因；克隆；序列分析；

Keywords:Banana, Hevea brasiliensis, cat gene, cloning, sequence analysis,

摘 要 ：根据GenBank上已有的植物cat基因序列，设计一对兼并引物。在海南岛采集香蕉、巴西橡胶、黄灯笼辣椒、菠萝、甘蔗、番木瓜等作物的叶片并提取总RNA，反转录成cDNA，PCR进行同源克隆。结果获得了巴西橡胶cat-1和香蕉的cat-2基因，而未获得其它4种作物cat相关基因。序列分析发现，巴西橡胶cat-1和香蕉的cat-2基因开放阅读框(ORF)都为1 479 bp，编码492个氨基酸，GenBank登陆号分别为HQ660587和HQ660588。生物信息学软件分析巴西橡胶CAT-1和香蕉CAT-2蛋白的三级结构分别与Exiguobacteriu Oxidotolerans(PDB code：2j2mA0)和Pseudomonas Syringae(PDB code：1m7sA)相似。构建系统发育树表明巴西橡胶CAT-1与蓖麻CAT-1氨基酸序列(Ricinus communis:XP_002521709.1)同源性最高，为92.1%；香蕉CAT-2与油棕CAT-2氨基酸序列(Elaeis guineensis:ACF06566.1)同源性最高，达90.9%。

Abstract:According to the existence of plant cat genes in GenBank, we designed a pair of degenerate primers, and amplified cat genes of banana, Hevea brasiliensis, yellow lantern pepper, pineapple, sugar cane, papaya from Hainan island. Ultimately, we cloned the rubber cat-1 and banana cat-2 genes, while the others not. Sequence analysis showed that open reading frame (ORF) of rubber cat-1 and banana cat-2 genes were both 1 479 bp, and encoded 492 amino acids. GenBank accession number were HQ660587 and HQ660588. Using bioinformatics software, the tertiary structures of rubber’s CAT-1 and banana’s CAT-2 proteins were predicated, which were similar to Exiguobacteriu Oxidotolerans (PDB code：2j2mA0) and Pseudomonas Syringae (PDB code：1m7sA), respectively. Phylogenetic analysis indicated that Hainan H.brasiliensis CAT-1 possessed the highest homology with Ricinus communis CAT-1 (XP_002521709.1), while banana CAT-2 was the highest homology with Elaeis guineensis CAT-2 (ACF06566.1).

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