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作 者 ：赵恩泽;金时;魏作富;魏巍;付玉杰;*

期 刊 ：植物研究 2011年 31卷 4期 页码：499-502

关键词：木豆叶；总黄酮；比色法；

Keywords:pigeon pea leaves, total flavonoids, colorimetric assay,

摘 要 ：采用比色法测定木豆叶中的总黄酮含量。对三氯化铝比色法、硼酸—柠檬酸比色法和硝酸铝比色法3种测定方法进行比较，确定了硝酸铝比色法为木豆叶总黄酮的最佳测定方法，该方法稳定性、精密度和重现性好，其RSD分别为2.1%、0.9%和2.3%，应用该方法测定木豆叶总黄酮含量为15.65 mg·g^-1 DW。本方法适用于木豆叶或其制剂中总黄酮的质量分析检验，为木豆叶中黄酮的研究开发提供了质控依据。

Abstract:AlCl^-₃, boric acid-citric acid-and Al(NO)₃-based colorimetric assays were compared to determine the total flavonoids in pigeon pea leaves. The results indicated that Al(NO)₃ colorimetric assay was the most appropriate for the determination of total flavonoids in pigeon pea leaves. The stability, precision and repeatability were very good, with RSD being 2.1%(n=3), 0.9%(n=3) and 2.3%(n=5), respectively. The content of total flavonoids in pigeon pea leaves was 15.65 mg·g^-1 DW. The method is appropriate for the quantitative determination of total flavonoids in pigeon pea leaves and their preparations. It can be referenced for the quality control of pigeon pea leaves.

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