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作 者 ：范阿棋;;毛娟;;刘思妍;;张俊莲;;王蒂;*;白江平;

期 刊 ：植物研究 2013年 33卷 (3)期 页码：294-301

关键词：马铃薯；StSnRK2.2基因；

Keywords:Solanum tuberosum, StSnRK2.2, cloning, bioinformatic analysis,

摘 要 ：以马铃薯‘陇薯3号’（Solanum tuberosum）为试材，采用RT-PCR方法从马铃薯cDNA中克隆得到一个SnRK(Sucrose nonfermenting 1related protein kinase)基因，命名为StSnRK2.2，提交GenBank注册，注册号为JX280912。通过生物信息学分析，该基因开放阅读框全长1 020 bp，编码339个氨基酸，蛋白质分子量为38.33 kDa，等电点为5.93，α-螺旋43.36%，延伸链16.22%，β-折叠6.78%，无规则卷曲33.63%，无信号肽、不跨膜，是一个膜内蛋白。亚细胞定位分析预测主要存在于细胞核中。氨基酸序列同源性比较发现，马铃薯StSnRK2.2基因与其他目前已报道的抗逆效果显著的SnRK2家族基因具有较高同源性，同烟草PK11-C5基因的同源性最高，相似性达90.56％，在系统进化树中处于同一进化分枝上。并通过分析得到该基因具有13处丝氨酸磷酸化位点、4处苏氨酸磷酸化位点和4处酪氨酸磷酸化位点，可能与植物体内Ser/Thr蛋白激酶有关，因此，推测该基因属于SnRK2基因家族成员，可能在参与逆境胁迫反应、增强植物的抗逆性中起着重要作用。

Abstract:The full-length cDNA sequence of SnRK2.2 in Solanum tuberosum (longshu 3) was successfully cloned by PCR. The gene was named StSnRK2.2 and has been submitted to Genbank with the accession number (GI:404435144). The bioinformatic analysis showed that the StSnRK2.2 has a complete opening reading frame (ORF) sequence contains 1 020 bp, which encoding 339 amino acids. The predicted protein molecular weight is 38.33 kDa and the theoretical pI is 5.93. The secondary structure of StSnRK2.2 indicated that about 43.36% of amino acids formed into alpha helix, 16.22% in extended strand, 6.78% in beta turn and 33.63% in random coil. There are no obviously classical signal peptide and transmembrane domain. The subcellular analysis indicated that the protein might play roles in the cell nucleus. Polygenetic analysis showed that the sequence is very close to the SnRK2 member in tobacco, the similar coefficient is as high as 90.56%. According to the amino acids sequence analysis, as a Ser/Thr protein kinase, the protein contains thirteen potential serine phosphatase sites, four threonine sites and four tyrosine sites. The results suggested that the gene product of StSnRK2.2 might be an important protein kinase related to the drought/stresses tolerance of potato plant.

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