Abstract:The banana seedlings were treated with brassinolid (BR) of different concentrations under the cold-stressed condition. Comparing to the control, the treatment with certain concentration may obvious reduce the electrolyte leakage rate, increase SOD activity, enhance the soluble protein content at a certain extent, slow down the MDA content change, distinctly enhance soluble sugar content, slow down the area of wither and mortalityrate as well as delay the chlorophyll degraded speed of the banana seedling leave. These changes of metabolite have played a key role and important function in enhancing cold-stressed period resistance of banana seedlings. The concentration with the best protected effect is 0.9 mg·L-1.