---

期 刊 ：植物保护学报 2007年

关键词：吸水链霉菌；分类鉴定；生防作用；灰霉病；

Keywords:Streptomyces hygroscopicus, taxonomic identification, biocontrol effect, gray mold,

摘 要 ：从京郊菜园土壤中分离到一株拮抗放线菌,编号为A03。为了科学评价该菌株在植物病害生防中的应用潜力,采用平板对峙培养法和温室盆栽防治法测定了其抑菌活性,并通过形态学、化学和分子分类的方法研究其分类地位。结果表明,该菌株对供试的多种植物病原真菌均有抑制作用,其中对果蔬灰霉病菌、番茄早疫病菌、小麦纹枯病菌和瓜果腐霉的抑制作用较强,平板抑菌带宽度达11.0~12.0mm。温室防病试验结果表明,其发酵上清液对甜椒灰霉病和番茄灰霉病具有良好的生防作用,防治效果分别为84.97％和79.51％。根据其菌株形态特征、培养性状、生理生化特性、细胞壁化学组分及16S rDNA序列分析,将菌株A03归入链霉菌属,并鉴定为吸水链霉菌Streptomyces hygroscopicus。

Abstract:An antagonistic actinomyces strain A03 was isolated from vegetable soil in suburb of Beijing. In order to evaluate scientifically the applied potential of the strain for the biocontrol of plant diseases, the fungistatic activity of A03 was determined by confronting incubation on PDA plates and control test to the potted plants in greenhouse, and the taxonomic identification of the strain was carried out with morphological, chemical and molecular methods. The results showed that the strain A03 displayed a broad-spectrum inhibiting activity to all the 15 plant pathogenic fungi tested. Especially, the inhibition zones out to the width of 11.0-12.0mm were presented by the strain against 4 ones of the pathogens, Botrytis cinerea, Alternaria solani, Rhizoctonia cerealis and Pythium aphanidermatum. The biocontrol effect of strain A03 to gray molds of pepper and tomato reached respectively 84.97% and 79.51% in greenhouse. According to the characteristics of morphological, cultural, physiological and biochemical, chemical components of cell wall, as well as 16S rDNA sequence, the strain A03 was identified as Streptomyces hygroscopicus.

---