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期 刊 ：植物保护学报 2008年

关键词：灰葡萄孢菌；激活蛋白PEBC2；纯化；生物活性；

Keywords:Botrytis cinerea, activator protein PEBC2, purification, bioactivity,

摘 要 ：通过沸水浴、离心、阴离子交换层析等方法,从灰葡萄孢菌Botrytis cinerea BC-4-2-2-1菌丝体中分离纯化出一种激活蛋白,命名为PEBC2,经SDS-PAGE银染显示呈单一条带,相对分子量约为14kD。该激活蛋白能显著提高小麦种子的发芽率,促进小麦幼苗生长,增强小麦的抗旱性和番茄对灰霉病的抗性。经激活蛋白处理后小麦发芽率从75.00%提高到98.33%,处理7、9、11天时株高分别比对照增加了17.07%、23.05% 和17.19%,幼苗抗旱综合系数从36.53提高到57.45;该激活蛋白对番茄灰霉病的诱抗效果达60.09%。

Abstract:An activitor protein named as PEBC2 was isolated and purified from the hyphae of Botrytis cinerea by boiling water bath, centrifugation, ultra-filtration and anion exchange chromatography, etc. The activator protein appeared as a single band corresponding to molecular weight of approximately 14kD on SDS-PAGE with silver staining. This protein can obviously promote seed germination, seedling growth and drought tolerance of wheat, it can also induce tomato to gain resistance to gray mould. By treating with this protein, the germination rate of wheat seed increased from 75.00% to 98.33%, the plant height of wheat increased by 17.07%, 23.05%, 17.19% in 7, 9, 11 days respectively, the comprehensive drought-resistance coefficient of wheat seedling improved from 36.53 to 57.45. This protein’s effect of induced resistance to gray mould of tomato reached 60.09%.

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