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期 刊 ：植物保护学报 2009年

关键词：HIA-1；生物活性；细胞相对存活率；过氧化物酶；超氧化物歧化酶；

Keywords:HIA-1, bioactivity, comparative survival rate of cells, peroxidase, superoxide dismutase,

摘 要 ：采用培养皿生物测定方法研究化合物O,O-二甲基-1-(2,4-二氯苯氧基乙酰氧基)呋喃甲基膦酸酯(试验代号HIA-1)的生物活性及其对苘麻部分生化指标的影响。结果表明,化合物HIA-1在0.05~10mg/L处理剂量下,对高粱、稗草、黄瓜、萝卜、苘麻和油菜的根、芽生长有不同程度的抑制作用,其中黄瓜、萝卜、苘麻和油菜较为敏感,10mg/L处理后,根长、芽长抑制率分别在86.2%和88.2%以上;0.01mg/L处理后对根、芽生长有明显的促进作用。0.1mg/L处理后,苘麻幼根细胞存活率超过100 %,表现出激活作用;随着浓度升高,细胞存活率逐渐降低。1000mg/L HIA-1处理时,苘麻幼苗及幼根SOD活性分别约为对照的50%和30%;当浓度分别为0.1和1mg/L时,幼苗和幼根SOD活性高于对照。10~1000mg/L处理时幼苗和幼根POD活性低于对照,1000mg/L时约为对照的58%和40%;低浓度时POD活性高于对照, 1mg/L时幼苗和幼根POD活性均约为对照的130%。

Abstract:The bioactivity of the novel compound, (Symbol:HIA-1) and the influence on some biochemical indexes of Abutilon theophrasti were studied. The result showed that HIA-1 had different degree inhibitory effect to Sorghum moench, Echinochloa crusgalli, Cucumis sativus, Raphanus sativus, Abutilon theophrasti and Brassica campestris from 0.05mg/L to 10mg/L. Thereinto, Cucumis sativus, Raphanus sativus, Abutilon theophrasti and Brassica campestris were more sensitive, and the inhibitory rates of radicle and bud length were above 86.2% and 88.2% after treatment at 10mg/L. But the radicle and bud were promoted at 0.01mg/L. The cells comparative survival rate of radicle, which was reduced followed with increase of concentration, was above 100% at 0.1mg/L, representing simulative effect in A.theophrasti. Superoxide dismutase (SOD) activities of seedling and radicle of A.theophrasti were about 50% and 30% of control at 1000mg/L respectively, which were more than the control at 0.1 and 1mg/L. Peroxidase (POD) activities of seedling and radicle of A.theophrasti were lower than the control activities from 10 to 1000 mg/L, with 58% and 40% of those of the control at 1000mg/L, but higher than the control activities at lower concentrations with both about 130% of those of the control at 1mg/L.

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