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期 刊 ：植物保护学报 2011年

关键词：马铃薯；

Keywords:Alternaria augustiovoide, Echinochloa crus-galli, tenuazonic acid, lipoxygenases, lipid peroxidation,

摘 要 ：噻虫嗪和吡虫啉是具有触杀、内吸和胃毒活性的新烟碱类杀虫剂,对植食性害虫效果显著,近年来已成为新农药创制领域研究的热点。马铃薯甲虫Leptinotarsa decemlineata (Say)是国际公认的毁灭性检疫害虫,以成、幼虫取食茄科植物茎叶、花蕾和叶芽等部位。一般田块减产30%~50%,严重地块达90%以上。目前,我国马铃薯甲虫仅在新疆马铃薯种植区发生,但对其周边省份马铃薯生产构成威胁。本试验选用噻虫嗪和吡虫啉于马铃薯播前进行一次性拌种,探讨出苗90天后的叶片对马铃薯甲虫幼虫生长发育的影响。

Abstract:In order to know the action of tenuazonic acid (TeA) produced by Alternaria augustiovoide strain AAEC05-3 on Echinochloa crus-galli (L.), the effect of TeA on lipid peroxidation of E.crus-galli leaf was studied by leaf segment method in vitro. The result showed that low concentration TeA did not affect the membrane permeability and lipid peroxidation of E.crus-galli leaf, but the membrane permeability and lipid peroxidation increased significantly in 24 h after treatment with high concentration of TeA. When E.crus-galli leaves were treated with 100μmol/L TeA, the relative conductivity and malondialdehyde (MDA) content increased 28.21% and 63.58%, respectively. After the treatment of 100μmol/L TeA, lipoxygenases (Lox) activity of leaf increased 61.59%, meanwhile, the content of unsaturated fatty acids decreased dramatically. The content of linoleic acid and linolenic acid decrease 19.56% and 17.20% in 100μmol/L TeA treatment, respectively. After the treatment of 100μmol/L TeA, the fatty acid hydroperoxides (FAH) was largely accumulated in E.crus-galli leaf. The results indicated that TeA could induce the lipid peroxidation of E.crus-galli leave. It is deduced that the lipid peroxidation induced by TeA could be Lox-mediated lipid peroxidation.

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