---

期 刊 ：植物保护学报 2009年

关键词：丽蚜小蜂；Wolbachia；生殖；适应性；

Keywords:Encarsia formosa, Wolbachia, reproduction, fitness,

摘 要 ：通过喂食含有盐酸四环素的蜂蜜水抑制丽蚜小蜂体内Wolbachia的活性,并研究以烟粉虱为寄主时Wolbachia内共生菌对丽蚜小蜂生殖力、寿命和后代性别的影响。结果表明:抗生素处理后丽蚜小蜂产生雄性后代,雄性后代所占比例与抗生素浓度呈显著正相关(r=0.322, P<0.01)。在1mg/mL抗生素浓度下丽蚜小蜂产生的寄生蛹数(黑蛹数)为68.1 ±8.9,较对照的49.1±4.5显著增加;而雌成蜂寿命与对照无显著差异(抗生素处理15.56±1.55,对照12.50±0.88),且从第4天开始产生的后代全部为雄性。说明Wolbachia的共生对丽蚜小蜂的生殖力具有一定的负面影响,Wolbachia调控宿主生殖的强度与其密度有关。

Abstract:Using various doses of antibiotic tetracycline hydrochloride, the effect of Wolbachia on the offspring production, longevity and the sex ratio of the parasitoid wasp Encarsia formosa were measured. Males induced by feeding antibiotic to their mothers in all treatments. Following antibiotic treatments, the percentage of males rises at low dose of tetracycline and drops again. The total sex ratio of offspring produced by treated mothers was positively correlated to the doses of antibiotic (r=0.322, P<0.01). At the concentration of 1mg/mL the treated females produced more offspring than the control (treatment group 68.1±8.9, control group 49.1±4.5), but the difference in longevity was not significant (treatment group 15.56±1.55, control group 12.50±0.88). It concluded that: Wolbachia has a negative impose on E.formosa. The influence of Wolbachia depends on its density.

---