The response of mixed stage cultures of three most tolerant insect species, Sitophilus oryzae, Tribolium castaneum and Rhyzopertha dominica to ethyl formate
Abstract:In order to keep the ethyl formate (EtF) toxicity results from being enhanced by carbon dioxide (CO2) and obtain more accurate toxicity data of EtF, fumigations were conducted using flow-through fumigation system (FTFS) to investigate the response of mixed stage cultures of the most tolerant insect species, Sitophilus oryzae (L.), Rhyzopertha dominica (F.) and Tribolium castaneum (Herbst) to a range of EtF doses. It was found that high control (>95.96% mortality) of mixed stage cultures of T.castaneum and R.dominica at 25℃ and 70% RH for the 6h exposure to 43.34, 80.72, 99.08, 117.74 and 146.49mg/L. Good control of mixed stage cultures of S.oryzae was achieved in 6h exposures at 25℃ and 70% RH to 80.72, 99.08, 117.74 and 146.49mg/L ethyl formate, but poor control of S.oryzae pupae. This study will be of significance for insect pest control due to replicate the response of mixed stage cultures of three most tolerant insect species in practice.