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期 刊 ：植物保护学报 2006年

关键词：枯草芽孢杆菌；离子注入；诱变筛选；抑菌防病；

Keywords:Bacillus subtilis, ion implantation, mutation screen, inhibiting pathogenes and controlling disease,

摘 要 ：枯草芽孢杆菌生防菌Bs-916是一种在水稻病害防治中发挥作用的生防因子,为进一步提高其拮抗性能,获得生防效果更好的高效菌种, 利用不同剂量的N⁺对Bs-916进行离子注入处理, 在1 300个诱变处理的菌株中,经过初筛、复筛和定量筛选,筛选到11株拮抗性能比出发菌Bs-916提高10%以上的菌株,对筛选出的高效突变菌株进行室内抑菌和田间防病测定。结果表明,离子注入生防菌Bs-916的最适剂量为150~250×2.6×10¹³N⁺/cm²;突变菌株的抑菌拮抗带宽提高率比出发菌株Bs-916高4.3%~30.7%;防病效果比Bs-916高3.2%~19.1%。

Abstract:Bs-916 of Bacillus subtilis was an important biocontrol factor in control of rice disease. In order to improve the growth rate and antagonistic ability of the strain, and to obtain high-efficiency strains, N⁺ of different doses was implanted into the biocontrol bacterium Bs-916. Of 1 300 strains mutation treated, 11 strains antagonisnic ability increased more than 10% that of Bs-916, by the first and the second screening, and fix quantify screening. Efficiency of inhibiting pathogens of the screened strains in vitro and controlling disease in the fields were measured. The results indicated that the optimum dose of ion implantation for the Bs-916 was 150×2.6×10¹³ N⁺/cm² to 250×2.6×10¹³N⁺/cm²; inhibition zone of the screened strains against Rhizoctonia solani were increased by 4.3% -30.7% in vitro; the control effect of rice sheath blight increased by 3.2% -19.1% over that of Bs-916.

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