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期 刊 ：植物保护学报 2005年

关键词：木霉；嘧霉胺；耐药性；突变体；

Keywords:Trichoderma, pyrimethaniol, tolerance, mutant,

摘 要 ：选育对杀菌剂具有抗性的生防菌株是生物防治研究的重要内容之一。通过药剂选择培养基及紫外光诱导获得对嘧霉胺具抗性的突变体KTm-1、KTm-2。研究结果显示,与亲本菌株相比,抗性突变体的耐药性提高20倍以上。抗性菌株在无药培养基上连续转代8次,耐药性未见下降。活体拮抗测定表明,耐药菌株的抑菌能力略高于亲本菌株。PAGE显示抗性突变体KTm-1的可溶性蛋白谱带比其亲本菌株多4条,其中 R_f =0.64的蛋白谱带是两个耐药菌株的特异性谱带,可视其为耐药相关蛋白。经Sephadex G-100柱层析纯化,SDS-PAGE测定耐药蛋白分子量为44.1KDa。

Abstract:Induce of biocontrol strain tolerance of Trichoderma to chemical fungicides was studied. Two mutants tolerant to pyrimethanil were obtained by UV-light induction on PDA amended with pyrimethaniol from a wild Trichoderma strain. Tolerance level was over 20 times in comparison with that of the mother strain. The mutants could maintain their tolerance after 8 times of transfers on the fungicide free PDA. The soluble protein profile bands of tolerant strains were higher than that of their original sensitive strains among which, the tolerant strains owned the soluble protein profile band of R_f =0.64. But it was not shown in sensitive strains, and it is called related tolerant protein. This protein was purified from KTm-1 by Sephadex G-100 gel filtration. The molecular weight of tolerant protein was determined to be 44.1 KDa. Two tolerant strains kept the ability of antagonism against Botrytis cinerea in vitro and vivo.

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