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作 者 ：林 燕1, 洪艳艳1, 史庆华1, 2*, 王秀峰1, 2, 魏 珉1, 2, 杨凤娟1, 2

期 刊 ：植物营养与肥料学报 2010年 16卷 5期 页码：1294-1298

关键词：黄瓜；

Keywords:cucumber, sodium nitroprusside(SNP), NaHCO3 stress, enzyme activity,

摘 要 ：采用溶液培养方法，研究了外源NO供体硝普钠（sodium nitroprusside, SNP）对NaHCO3胁迫下黄瓜幼苗的缓解效应。结果表明，100 μmol/L SNP能有效减轻30 mmol/L NaHCO3对黄瓜植株地上部和地下部生长的抑制，提高了NaHCO3胁迫下黄瓜叶片叶绿素和类胡萝卜素含量、净光合速率（Pn）以及荧光参数Fv/Fm和ΦPSⅡ。HaHCO3胁迫显著抑制了氮代谢相关酶硝酸还原酶（NR）、谷氨酰胺合成酶（GS）和谷氨酸合成酶（GOGAT）的活性; 外加SNP处理明显缓解了NaHCO3对它们活性的抑制。

Abstract:A solution culture experiment was conducted to study the effects of SNP on plant growth, photosynthesis, antioxidant enzyme activity and nitrogen metabolism in cucumber seedlings under NaHCO3 stress. The results indicated that 100 μmol/L SNP promoted the growth of cucumber seedlings and increased chlorophyll and carotenoid contents, net Photosynthetic rate (Pn), Fv/Fm and ΦPSⅡunder NaHCO3 stress. Compared with control, the activities of nitrate reductase (NR), glutanine synthetase (GS) and glutamate synthase (GOGAT) were significantly inhibited by NaHCO3 stress and SNP increased their activities.

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