Abstract:The effect of excessive Fe2+ on plant growth and physiological characters were studied in our previous research. For a further investigation on the poisoning mechanism Fe2+ , the ultrastructure of the leave cells of Glycine max ( L. ) Merrill was observed in this paper. The change of ultrastructural under excessive Fe2+ stress for two soybean varieties, named as "Zhechun No.3" and "1601" separately, was described. The results showed: with a increase of Fe2+ concentration, the ultrastructural damage on the "Zhechun No. 3" leave cells unceasing aggravated; huge starch grains appeared in chloroplasts; granas became disordered; some cristae of mitochondria decreased or was chaos. As the results, the chloroplast and mitochondria membrane disrupted; and some fragments of membrane system were found in vacuoles of leaf cytoplasm. The plasmalemma folded entad forming wave shape. When leaf cells suffered low-middle degree Fe2+ poison, the concentration of Fe2+being 100 mg/kg and 300 mg/kg, the chloroplast structure of "1601" was injured slightly. However, under high concentration Fe2+ poison, the accumulation of starch grains in the chloroplast, disruption of chloroplast membrane disrupted; disintegration of mitochondria cristae resulted in the vacuolization of mitochondria appeared in the leaf cells of ‘1601‘ . The results indicated that: excessive Fe2+ could injure the membranous structures of cells, among which the injury on chloroplast and mitochondria was more serious. Excessive Fe2+ caused the accumulation of starch grains in the chloroplast of leaf cells. The leaf cells ultrastructure of two soybean varieties had different response to Fe2+ poison, "Zhechun No.3" soybean was more sensitive to Fe2+ .