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作 者 ：鲁翠涛1;李合生2;王学奎2

期 刊 ：植物营养与肥料学报 2002年 8卷 1期 页码：110-114

关键词：钙；小麦；氮同化；关键酶；蛋白质磷酸化；

Keywords:calcium, wheat, key enzyme of nitrogen assimilation, protein phosphorylation,

摘 要 ：为探讨氮素同化机理 ,小麦幼苗经溶液培养 (培养液中不含Ca²⁺)至二叶一心后 ,在培养液中分别设置不同浓度的Ca²⁺ 、Ca²⁺专一性螯合剂EGTA以及Ca²⁺通道阻塞剂LaCl₃进行处理 (进行EGTA和LaCl₃处理时 ,培养液中Ca²⁺浓度为 1mmol/L)。结果表明 ,氮同化关键酶硝酸还原酶 (NR)、谷氨酰胺合成酶 (GS)活性同Ca²⁺/CaM依赖性蛋白激酶 (Ca²⁺/CaM PK)活性之间存在明显的相关性。说明钙作为第二信使所介导的信号转导中 ,蛋白质可逆磷酸化可能参与了对氮素同化的调节作用。

Abstract:The mechanism of nitrogen assimilation on green wheat seedlings through adding different concentration of Ca²⁺ 、Ca²⁺ specific chelator EGTA and blocker of Ca²⁺ channel LaCl₃ in nutrient solution (when treated use of EGTA or LaCl₃, there was 1mmol/L Ca²⁺ in nutrient solution) was studied. The results showed that there was significant correlation between the key enzymes of nitrogen assimilation (nitrate reductase, NR; glutamine synthetase, GS) and Ca²⁺/CaM dependent protein kinase (Ca²⁺/CaM PK).It indicated that reversible protein phosphorylation could take an important part in regulating the signal transduction pathway of calcium.

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