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Phylogeny and Interspecific Relationship of Lilium Section Leucoliron Based on Three Gene Sequences

基于3种基因序列对百合属Leucolirion组的系统发育与种间关系研究



全 文 :书!"#$%&
,2016,36(8):1541-1550
犃犮狋犪犅狅狋.犅狅狉犲犪犾.犗犮犮犻犱犲狀狋.犛犻狀.
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H I:stuvw`xFyz{|fg,}u]yz~`KLk[MN]K:;\€‚。ƒ:;,„
犐犜犛、cpDNA(狋狉狀犞狀犱犺犆、狆犲狋犔狆狊犫犈)…†‡ˆ‰,Š犃犓犜‹3Œ,ŠŽ,‘uY’„!“”•`yz~6
Œ–—Z˜™48š›4…GenBankœ`žŸ,€yz~犔犲狌犮狅犾犻狉犻狅狀 M¡¢KLk[{£]K:;。¤¥
¦§
:(1)犐犜犛¤¥¨©ª«`¬­,®¯犔犲狌犮狅犾犻狉犻狅狀 °Y±2Œ² ,subsect.犔犲狌犮狅犾犻狉犻狅狀6a³subsect.犔犲狌
犮狅犾犻狉犻狅狀6b。´µ¯¶"yz(犔.犺犲狀狉狔犻)³“byz(犔.狉狅狊狋犺狅狉狀犻犻)·¸¹subsect.犔犲狌犮狅犾犻狉犻狅狀6a,[º»yz
(犔.狊犪狉犵犲狀狋犻犪犲)、¼½¾yz(犔.狊狌犾狆犺狌狉犲狌犿)、¿Àyz(犔.犾犲狌犮犪狀狋犺狌犿)³ÁÂyz(犔.狉犲犵犪犾犲)‹4Œ{ÃÄ 
q犔犲狌犮狅犾犻狉犻狅狀 ;Å4~„犃狉犮犺犲犾犻狉犻狅狀 `xyz(犔.犫狉狅狑狀犻犻)[subsect.犔犲狌犮狅犾犻狉犻狅狀6bƱè,ǯÈ[2
Éyz
(犔.犾狅狀犵犻犳犾狅狉犿)、ÊËyz(犔.犳狅狉犿狅狊犪狀狌犿)‹ qÌ̠,dͱyz (sect.犔犻犾犻狌犿)。(2) £]K
Î
,犔犲狌犮狅犾犻狉犻狅狀 [yz `KLk]KÏ,ªR[Y’„Ð4`犃狉犮犺犲犾犻狉犻狅狀 ÑqÒÓZ˜,ÔÕR[犛犻
狀狅犿犪狉狋犪犵狅狀 `֗]Kז。(3),„ØÙÚÛz,Š³†‡ˆ‰,Š犃犓犜`6Œ–—{`ÜÝÞßàá,¶
"yz[“byz§à•â„犔犲狌犮狅犾犻狉犻狅狀 ãtuäåæç¾`4ŒZ˜。ØÙÚÛz,Šè³†‡ˆ‰,Š
褥¦§

º»yz

¼½¾yz

ÁÂyz³¿ÀyzBÜÝé1Φêë¢ìí`\î9

ÜÝ]KïY–

ðñò[yz~#$‘ó`ôkKLu]

õö¢${£ÜÝ÷ø[YN

EJK
:犔犲狌犮狅犾犻狉犻狅狀;KLk;犐犜犛;ØÙÚ,Š;†‡ˆ‰,Š;{£]K
LMNO$
:Q789 !PQRS:A
犘犺狔犾狅犵犲狀狔犪狀犱犐狀狋犲狉狊狆犲犮犻犳犻犮犚犲犾犪狋犻狅狀狊犺犻狆狅犳犔犻犾犻狌犿犛犲犮狋犻狅狀
犔犲狌犮狅犾犻狉狅狀犅犪狊犲犱狅狀犜犺狉犲犲犌犲狀犲犛犲狇狌犲狀犮犲狊
LAIShanpan,SHENChengjuan,ZHOUSongdong,HEXingjin
(KeyLaboratoryofBioResourcesandEcoEnvironmentofMinistryofEducation,ColegeofLifeScience,SichuanUniversity,
Chengdu610064,China)
犃犫狊狋狉犪犮狋:DespiteabundantgermplasmresourcesofwildliliesinChina,thestudyofphylogeneticandinter
specificrelationshipin犔犻犾犻狌犿 wasscarce.Inthisstudy,phylogenyofthesection犔犲狌犮狅犾犻狉犻狅狀Comber
whichincludesGenBankdataand48specimenscovering6closedrelatedliliesendemictoSouthwestChina
wasexploredbasedon犐犜犛,cpDNAandasinglecopynucleargene,犃犓犜.Theresultsshowedthat:(1)
inaccordancewithpreviousstudiesthatthesection犔犲狌犮狅犾犻狉犻狅狀Combershouldbedividedintotwosubsec
tion,subsect.犔犲狌犮狅犾犻狉犻狅狀6aandsubsect.犔犲狌犮狅犾犻狉犻狅狀6b,and犔.犺犲狀狉狔犻and犔.狉狅狊狋犺狅狉狀犻犻shouldbeclas
sifiedintosubsect.犔犲狌犮狅犾犻狉犻狅狀6a.Werevisesection犔犲狌犮狅犾犻狉犻狅狀including犔.狊犪狉犵犲狀狋犻犪犲,犔.狊狌犾狆犺狌狉犲
狌犿,犔.犾犲狌犮犪狀狋犺狌犿,犔.狉犲犵犪犾犲,犔.犺犲狀狉狔犻and犔.狉狅狊狋犺狅狉狀犻犻.犔.犫狅狑狀犻犻,whichoriginalybelongedtosec
tion犃狉犮犺犲犾犻狉犻狅狀wasplacedintosubsect.犔犲狌犮狅犾犻狉犻狅狀6b,alongwith犔.犳狅狉犿狅狊犪狀狌犿and犔.犾狅狀犵犻犳犾狅狉狌犿,
weestablishanewgroup,namedsection犔犻犾犻狌犿.(2)Asforinfragenericrelationships,section犃狉犮犺犲犾犻狉犻狅狀
whichmainlydistributesinJapanshowedasisterrelationshipwithsection犔犲狌犮狅犾犻狉犻狅狀.However,section
犔犻犾犻狌犿and犛犻狀狅犿犪狉狋犪犵狅狀showedacloserelationship.(3)Thegeneticdistancesamongaltaxaofchloro
plastcombinationand犃犓犜showedthat犔.犺犲狀狉狔犻and犔.狉狅狊狋犺狅狉狀犻犻distinctlydifferfromothermembers
ofsection犔犲狌犮狅犾犻狉犻狅狀.Phylogenetictreebasedonchloroplastcombinationdata,犃犓犜fragmentindicated
that犔.狊犪狉犵犲狀狋犻犪犲,犔.狊狌犾狆犺狌狉犲狌犿,犔.狉犲犵犪犾犲and犔.犾犲狌犮犪狀狋犺狌犿haveaverycloserelationship.Highge
neticsimilaritybetweenthesespeciesmaybeattributedtotheparticularmatingsystemof犔犻犾犻狌犿,leading
tolimitedgeneticvariationanddifferentiationamongspecies.
犓犲狔狑狅狉犱狊:犔犲狌犮狅犾犻狉犻狅狀;phylogeny;犐犜犛;chloroplastgene;singlecopynucleargene;interspecificrela
tionship
  yz~(犔犻犾犻狌犿 L.)ù~„yz+(Liliaceae
Juss.),úûüý110~115þ{,ÿ!Y’„""
#$%”•

su55{[1],&yz~#$`Äg
³Y’s
[2],
(s)!“”•Y’*±vw

Comber[3]+Ÿ13ŒÑh9ç³2ŒF,‘9¯y
z~-Y±7Œ 9Œ² 。(s,犔犲狌犮狅犾犻狉犻狅狀 
`GH‘.±Ø/F

01

¾±23ç

6Øë4
56

ƒ  7+Ÿ89:å;Yq¢2Œ²
 

Y â ± subsect.犔犲狌犮狅犾犻狉犻狅狀6a ³ subsect.
犔犲狌犮狅犾犻狉犻狅狀6b。ªR<=¢ÁÂyz(犔.狉犲犵犪犾犲)、
¿Àyz
(犔.犾犲狌犮犪狀狋犺狌犿)、º»yz(犔.狊犪狉犵犲狀狋犻
犪犲)³¼½¾yz(犔.狊狌犾狆犺狌狉犲狌犿),89±>å,‘
uY’„s!“”•

ÕR?ÊËyz
(犔.犾狅狀
犵犻犳犾狅狉狌犿)、2Éyz(犔.犳狅狉犿狅狊犪狀狌犿)³@ABy
z
(犔.狆犺犻犾犻狆狆犻狀犲狀狊犲)‹ q,89±äå,GHY
’„sC8DE…2ɔ•

}FG°H%[Y
6KL%:;`I¸

ƒ `-Y… 7Z˜¹
¢MÃJ`KL[M

*N
,Nishikawa‹[45]… Hayashi‹[6]€yz
~`Y6KL%:;¦§
,犔犲狌犮狅犾犻狉犻狅狀 O&Ì
†K

ƒ 7PB2ŒÖ—]KÏ`Y¨。Gao
‹
[7]
€yz~`‰5:;àá

ÑhÎQ±äåæ
ç¾`Z˜

+ŸR£FÚ`u0¯犔犲狌犮狅犾犻狉犻狅狀 
Yq2Œ² ,Ss!“”•`ÁÂyz、¿Ày
z

º»yz³¼½¾yzTuR£FÚUY±¢
̲ 

ÔV?yz
(犔.狑犲狀狊犺犪狀犲狀狊犲)、WXy
z

ÊËyz³2ÉyzYZuðä[U·„ò 

ðì­]¨©¢Comber[3],„89:å
€ƒ `-Y

}\„ComberB1949^€犔犲狌
犮狅犾犻狉犻狅狀 `-Y,_¸¢`aÕb¦`ÌZ˜。
–^b

×ccd7`KLkl´eyz~`
YZKLfgh
。Lee‹[8]€yz~83{14÷
{M¡犐犜犛´èYi,犔犲狌犮狅犾犻狉犻狅狀 UYq2Œ†
j`Y¨
,GroupI³ GroupVI。Gao‹[2,9],„
犐犜犛³犿犪狋犓 €yz~`KLk:;¤¥àá,
¶"yz
(犔.犺犲狀狉狔犻)[subsect.犔犲狌犮狅犾犻狉犻狅狀6aÆ
±Ã¨

vw¢ðò `${ q

kƒ² [Y
’„Ð4`犃狉犮犺犲犾犻狉犻狅狀 Ñq¢ÒÓZ˜]K;
subsect.犔犲狌犮狅犾犻狉犻狅狀6bY<=¢xyz(犔.犫狉狅狑
狀犻犻)、WXyz(犔.犪狀犺狌犻犲狀狊犲)、V?yz、ÊËy
z

2Éyz³@AByz‹6Œ{,†jƱÃ
¨

[subsect.犔犲狌犮狅犾犻狉犻狅狀6a`֗]KÏ。
Du‹[10][´¢lª*c`ITSMNè,¤¥¨©
m¶"yz³“byz
(犔.狉狅狊狋犺狅狉狀犻犻)·¸¹sub
sect.犔犲狌犮狅犾犻狉犻狅狀6as,犔犲狌犮狅犾犻狉犻狅狀 UKn±2
ŒY¨

)Î`:;¤¥¦§
,犔犲狌犮狅犾犻狉犻狅狀 &O
)*`

oŸ(”­Y’p›)…°H%

Y6KL
%qŸ

ƒ rHU°Y³s­

lª€¶"yz³“byz`KLtuvZu
̻w

*NComber[3]+ŸÑh‘.¯xR·¸
犛犻狀狅犿犪狉狋犪犵狅狀 ,}Y6KL%`:;[ÑhYZ
¤¥ëê¢yc`zø

È{`KLturHlÌ
|}

ÔÁÂyz

¿Àyz

º»yz³¼½¾y
z`ÑhïY\–

r±äåæç¾

}{£ü~
O§à

Y6KL%`¤¥àáKLkè`€m
¨©Oí

Y¨ãPBc‚`1¡¨
[2,10],
Šƒ
{£]KrHMÃJ:;³„…

4noBª«:
;`,.Î

O†c‚`x‡ˆ‰³›

ÎŠ
‘S„s!“”•`Ñh\î

Y6KLΖ—
`6ŒZ˜(‹1),,„ØÙÚ,ŠŽ狀犱犺犆狋狉狀犞
2451 ! " # $ % &                   36Œ
A、B.¶"yz³“byz(፽厏ç¾U);C~G.ÁÂyz、¿Àyz、¼½¾yz、º»yz³>yz(áäå
23ç¾U
);H、I.¶"yz、“byz>å89
‹1 ‘„4:;`yz#’Ñh‹
A,B.犔.犺犲狀狉狔犻and犔.狉狅狊狋犺狅狉狀犻犻withorangeTurksCapshapedflowers;C-G.犔.狉犲犵犪犾犲,犔.犾犲狌犮犪狀狋犺狌犿,犔.狊狌犾狆犺狌狉犲狌犿,
犔.狊犪狉犵犲狀狋犻犪犲and犔.犾犲狌犮犪狀狋犺狌犿var.犮犲狀狋犻犳狅犾犻狌犿withwhitetrumpetshapedflowers;H,I.Purplebulbsof犔.犺犲狀狉狔犻and犔.狉狅狊狋犺狅狉狀犻
Fig.1 Imagesof犔犻犾犻狌犿usedinthisstudy
³狆犲狋犔狆狊犫E、‰“Ú,Š犐犜犛Ž…†‡ˆ‰,
Š犃犓犜 Ž,€(KLk[{£]KM¡¢Y
i:;

4:;Z˜``aq”¾c•–

k”7
89ñQ—‘

ÿ!˜‘„ÜÝk{

¾™šk‹›
œ

ŠƒMðž{`KLk]K`:;uŸ„
±(z­`[Ÿ‘ ¡­w,.

1 ¢£³^¤
1.1 TUVW
4:;I2013~2014^¥Mx‡ˆ‰)
…›¦§¨©Q

¶"yz

“byzGHY’„


«¬³¶"Ã%

™§¨¹10ŒR˜。ÁÂ
yz­œY’„Á®œ

B¯b

°±Ã%§¨¹
4ŒR˜。¿Àyz…(÷{>yz犔.犾犲狌犮犪狀
狋犺狌犿var.犮犲狀狋犻犳狅犾犻狌犿 ™5ŒR˜,º»yzGH
Y’„ab



™²11ŒR˜;Ô¼½¾yzY
’*±ÿ!

³“

«¬

ÿ!‹”´S

™§¨¹
18ŒR˜。4:;Ÿ‘µœ`,Š¶·)…I
GenBankœ`(¸yz~#$`,Š¶·¹€
º

€犔犲狌犮狅犾犻狉犻狅狀 `KLk[{£]KM¡¢
:;

O»¼½7

¾ŒR˜1~2ŒŒÚQ±¿
¦

¢£À¨PÁ

Âq34æ1。ÌÄ؍´
‘ÅÆÇÈÉÊËP

Âq34PÌBabc%#
$34Í
(SZ),¾š34`”­À¨PÁr‘Χ
GPS(GPSMAP62sc,Shanghai)ÏÐÑÒmt。
34518Ó       >?@,‹:,„3{,Š¶·€yz~犔犲狌犮狅犾犻狉犻狅狀 `KLk[{£]K:;
X1 TUYZ
Table1Sourceofmaterials

Taxon
R˜ÔÕ
Population
code
Y’”
Location
Öd

×d
Longitude(E)/Latitude(N)
†Ø5
Haplotype
Âq34
Voucherspecimen
¼½¾yz
犔.狊狌犾狆犺狌狉犲狌犿
ZY «¬ÙÚZunyi,Guizhou 106.706006/27.59674 H1 L2014071001
BJ «¬Û€Bijie,Guizhou 105.40495/27.59413 H1 L2014070901
GY «¬«ÜGuiyang,Guizhou 106.688921/26.438402 H1 L2014071101
XY «¬TÚXingyi,Guizhou 107.99379/26.57925 H1 L2014071301
XR «¬TÝXingren,Guizhou 105.1571/25.42444 H1 L201407140
AS «¬WÞAnshun,Guizhou 105.9923/26.27725 - L2014071501
DY ³“cßDayao,Yunnan 101.09337/25.85903 H19 DY20130630
WSCC ³“V? Wenshan,Yunnan 104.111893/23.402378 H19 WS2013070201
WSXC ³“V? Wenshan,Yunnan 104.4177778/23.39833333 H4 WS2013070204
WSDT ³“V? Wenshan,Yunnan 104.3344444/23.49888889 - WS2013070101
XC ³“!àXichou,Yunnan 104.492356/23.260358 - XC20130702
CJ ³“áÂChengjiang,Yunnan 102.966077/24.571428 - CJ20130704
JC ³“ÂbJiangchuan,Yunnan 102.866741/24.495123 - JC20130704
YM ³“âãYimen,Yunnan 102.181305/24.610336 H1(2) YM20130602
ES ³“ä?Eshan,Yunnan 102.4516667/24.16777778 H10,H11 ES20130707
JY ³“-ÜJinyang,Yunnan 103.255798/27.656657 H1 JY20130731
NJ ³““åNanjian,Yunnan 100.316718/24.838849 H1 NJ20130713
HD abæC Huidong,Sichuan 102.631764/26.514499 H22 HD20130717
º»yz
犔.狊犪狉犵犲狀狋犻犪犲
YB ab¿BYibin,Sichuan 102.31653/31.65945 H3,H6 L2014070701
YL ³“çèYiliang,Yunnan 104.47764/27.86067 H1 L2014070901
BX abéTBaoxing,Sichuan 102.8175556/30.36263889 H1,H5 L2014080201
EB abäêEbian,Sichuan 103.3372222/29.35583333 H13 EB20130805
MB abëê Mabian,Sichuan 104.0022222/29.35388889 - MB20130804
DJY abrÂìDujiangyan,Sichuan 103.9916667/31.04444444 H12(2) DJY20130626
LD abº»Luding,Sichuan 102.4055556/30.56972222 H1,H17 LD20130728
TQ abíúTianquan,Sichuan 103.2172222/30.11861111 H1,H7 TQ20130729
JFS lª-î?Jinfoshan,Chongqing 107.2197222/29.09111111 H6,H13 JFS20130724
TQLD abíúﺻTianquantoLuding,Sichuan 102.7675/30.02194444 H6(2) 20130826
EMS abäð?Emeishan,Sichuan 103.4211111/29.57472222 H18 EM20130615
ÁÂyz
犔.狉犲犵犪犾犲
WC ab¯b Wenchuan,Sichuan 103.8498/31.70248 H8 L2014062501
LX ab­±Lixian,Sichuan 103.84472/31.69307 H8 L2014062502
MX ab°± Maoxian,Sichuan 103.57199/31.46643 H8 L2014062404
HS abñé Heishui,Sichuan 103.3327/31.9828 H2 L2014062801
¿Àyz
犔.犾犲狌犮犪狀狋犺狌犿
BC ab"bBeichuan,Sichuan 104.16775/31.79763889 H21 L2014080601
QC abòbQingchuan,Sichuan 104.1696389/31.80230556 H20 L2014080701
WX óôV± Wenxian,Gansu 104.7469722/33.24283333 H15 L2014080901
PW ab1õPingwu,Sichuan 104.350575/32.74389 H23 Y2013080501
ZQ óôö÷Zhouqu,Gansu 104.47606/33.798314 - Y2013071502
¶"yz
犔.犺犲狀狉狔犻
KL «¬øùKaili,Guizhou 107.99389/26.57912 - L2014071201
FL lªúûFuling,Chongqing 107.5684167/29.49375 - L2014081801
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(heuristic
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2.2[20]Îef*zt`E5,P»*tu¿E5±
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(MarkovChainMonteClarlo,MCMC)Q|˜¡
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2.1 ,678de
‰,Š犐犜犛`žŸƒ„<`¢98/¶·,<
=犔犲狌犮狅犾犻狉犻狅狀 ³犃狉犮犺犲犾犻狉犻狅狀 GHq”)…S
„s`(¸yz~#$

º€Õ`犐犜犛,Š¶·
1´kd±621(615~639)bp,<`¢234Œ÷ø
tm

PÁtm135Œ,…21.74%,G+C `‚
62.1%。ØÙÚ,ŠŽ狆犲狋犔狆狊犫犈 ³狀犱犺犆狋狉狀犞
€6Œ–—{`18ŒŒÚM¡78I¶,kdYâ
±1192~1196bp³746~753bp,PÁtmYâ
±11³10Œ。?„,Š¶·`PÁtmu~,€
狆犲狋犔狆狊犫犈 ³狀犱犺犆狋狉狀犞 M¡¢†Ã‡9kdoo
(ILDoo),¤¥àáˆ/¶·ò‰QRz(犘>
0.5),Šk±1939~1946bp,` u23Œ÷øt
m

PÁtm21Œ(1.1%)。†‡ˆ‰,Š犃犓犜
€:;Z˜`47ŒŒÚM¡78,¶·1´kd±
1459bp,G+C`‚‹37.9%,÷øtm100Œ,_
ýPÁtm67Œ(4.6%)。
2.2 fghiNc
)cpDNAÛz,Š³†‡ˆ‰,Š犃犓犜 `
¶·±žŸ,.

Ÿ‘ MegaS0s`K2PE5€
4:;Z˜`6Œ–—{3ŒR˜`18ŒŒÚM
¡ÜÝÞßYi

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ÜÝÞ߃„¤¥àá
,6Œ–—{{£`ÜÝÞ
ߌ

1´ý±0.0044。º»yz、¼½¾yz、
ÁÂyz³¿Àyz`{£ÜÝÞß\î

±0.000
~0.002。¶"yz、“byz[4Œäåæç¾Z
˜`ÜÝÞßB0.006~0.008Ž£。†‡ˆ‰,
Š犃犓犜B{£`ÜÝÞß1´±0.0199,[ØÙ
Ú,Š`¤¥Zî

ª4Œ{{£ÜÝÞߌ,c
B0.001~0.007Ž£。¶"yz[“byz`
{£ÜÝÞß1´±0.006,[º»yz‹4Œ{
`ÜÝÞßB0.032~0.036Ž£。
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X2 6jkl5>5Dfghi
Table2 Geneticdistancesamongspeciesin6closerelatedspecies
{Species
º»yz
犔.狊犪狉犵犲狀狋犻犪犲
¼½¾yz
犔.狊狌犾狆犺狌狉犲狌犿
ÁÂyz
犔.狉犲犵犪犾犲
¿Àyz
犔.犾犲狌犮犪狀狋犺狌犿
“byz
犔.狉狅狊狋犺狅狉狀犻犻
¶"yz
犔.犺犲狀狉狔犻.
º»yz犔.狊犪狉犵犲狀狋犻犪犲  0.001 0.006 0.001 0.032 0.033
¼½¾yz犔.狊狌犾狆犺狌狉犲狌犿 0.000  0.007 0.001 0.033 0.034
ÁÂyz犔.狉犲犵犪犾犲 0.001 0.001  0.007 0.035 0.036
¿Àyz犔.犾犲狌犮犪狀狋犺狌犿 0.001 0.001 0.001  0.033 0.034
“byz犔.狉狅狊狋犺狅狉狀犻犻 0.006 0.006 0.006 0.006  0.006
¶"yz犔.犺犲狀狉狔犻 0.007 0.007 0.007 0.007 0.007 
$

€Î^±,„†‡ˆ,Š犃犓犜`ÜÝÞß,€7^±cpDNAÛzŽ`ÜÝÞß
Note:GeneticdistancesbasedonnuclearsinglecopyAKTareabovediagonal,andgeneticdistancesbasedoncpDNAcombinationarebe
lowdiagonal
2.3 ?@ABE?Nc
,„犐犜犛¶·[´` MP賈Øqè`¤
¥àá

ˆ{^¤[´`KLk葒¤[\î

:‹2µá,犔犲狌犮狅犾犻狉犻狅狀 UY±¢2ŒY¨,Y
â±subsect.犔犲狌犮狅犾犻狉犻狅狀6a³subsect.犔犲狌犮狅犾犻狉
犻狅狀6b,kð2ŒY¨`KLk]KŒÏ。sub
sect.犔犲狌犮狅犾犻狉犻狅狀6a<`¢º»yz、¼½¾yz、
ÁÂyz³¿Àyz‹4Œ{,¶"yz³“by
z[ƒ² Œ“”Ʊè

ƒY¨[犃狉犮犺犲犾犻狉犻狅狀
 Ñq¢ÒÓZ˜]K
(PP/BS,0.85/80)。sub
sect.犔犲狌犮狅犾犻狉犻狅狀6b<=¢2Éyz、ÊËyz,x
yz[ƒ² `ÆZ¤¥¹Œí`¨©
(PP/
BS,0.95/88),sŒY¨[犛犻狀狅犿犪狉狋犪犵狅狀 `KL
k]K×±R–

B{£]KÎ

4:;s`6Œ–—{Ñq¢
̌“`j”Y¨
,¨
©ª«`Y6KL:;¤
¥

¶"yz[“byzsYNëb

}xRŽ£
`{£]KO§P

º»yz

¼½¾yz

ÁÂy
z³¿Àyz‹4Œ{`aŒR˜žŸZu¹
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Q±äåæç¾`4Œ–—{ƱÃ
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6Œ–—Z˜[´NJ(O<`‡˜)KLkè
Bootstrapvalues>50%aremarked.Theletterplusnumber
behindspecificnamerepresentindividualsfrom
differentpopulations
Fig.3 Phylogeneticrelationshipsofneighborjoining
(nooutgroup)forchloroplastDNAcombination(狀犱犺犆
狋狉狀犞+狆犲狋犔狆狊犫犈)in6closerelatedtaxa
¨

¹Œí`¨©
(99%)。ÁÂyz³¿Ày
zœ*]p)Ʊè

}¨©Oí

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Y¨ã8

º»yz³¼½¾yz`{£]KOP
»

±1¡Y¨lŸBÃÄ

78I¶`Z˜OÃ

k‡Z˜`OQ,Š¶
·] òœ

µ)ZuO†a,ŠÛz´èbM
ÃJoqyz~ã`KLkYi

)?#犔.
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`KLkè`¤¥àá

‹4),º»yz、¼½¾
yz

ÁÂyz³¿Àyz4Œ{Œ“”Ʊ¢Ã
¨
(PP/BS,0.98/97),}È{`{£]K¡*O§
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(PP)³*c_ýèBootstrap¨©(BS),{Í՛¤[ž›Yâ¦áGenBank
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7Q
‹2 ,„犐犜犛¶·€犔犲狌犮狅犾犻狉犻狅狀 …\]Z˜`KLkYi
Bayesianposteriorprobabilities(PP)andparsimonybootstrap(BS)aregivenatnodes,respectively
Theletterplusnumberbackofspecificnamerepresentpopulationorindividualcodes.Thesameasbelow
Fig.2 Phylogenyanalysisofsection犔犲狌犮狅犾犻狉犻狅狀anditsrelatedtaxabasedon犐犜犛sequence
74518Ó       >?@,‹:,„3{,Š¶·€yz~犔犲狌犮狅犾犻狉犻狅狀 `KLk[{£]K:;
‹4 ,„†‡ˆ‰,Š犃犓犜€6Œ–—
Z˜`KLkYi
Fig.4 Phylogenyanalysisof6closerelatedtaxa
basedonthenuclearsinglecopysequence犃犓犜
Ø5Yi¤¥àá
,42/,Š¶·ê¢23{†
Ø5

¦1),I†Ø5¥¦‹Î§(‹5),†Ø5H1
&Y’*ÿ

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g)º»yz
³¼½¾yz`c8YR˜

™©†Ø5 H6、H13、
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,H2、H3、H4、H5、H7、H15、H17、H18、H21、
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3 … w
3.1 犔犲狌犮狅犾犻狉犻狅狀=>?@ABNc
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B4:;s oI¹¸¹Öº»
‹5 ,„†‡ˆ‰,Š犃犓犜€6Œ–—{[´`
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Thesizeofthecirclesindicatesthefrequencyofeachhaplotype.
Emptydotsindicatethatintermediatehaplotypeswerenot
detectedinourresearchorextinction
Fig.5 Relationshipswthinthehaplotypenetwork
for6closerelatedtaxa
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uäåæç¾`º»yz

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狅狀 `xyz[subsect.犔犲狌犮狅犾犻狉犻狅狀6bƱÃ
¨

BKLtuÎ[犛犻狀狅犿犪狉狋犪犵狅狀 `֗]K
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