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Overexpression of NtMTP1 Gene from Nicotiana tabacum Improved Zinc Tolerance of Tobacco

NtMTP1基因过表达对增强烟草Zn胁迫耐受性的研究



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JKLM :Q785;Q786 !NOPQ:A 犗狏犲狉犲狓狆狉犲狊狊犻狅狀狅犳犖狋犕犜犘1犌犲狀犲犳狉狅犿犖犻犮狅狋犻犪狀犪狋犪犫犪犮狌犿 犐犿狆狉狅狏犲犱犣犻狀犮犜狅犾犲狉犪狀犮犲狅犳犜狅犫犪犮犮狅 RANQiao1,WUChanjuan1,2,GAOYongfeng1,TANGYunlai1,2, HUANGCaizhi1,LIUJikai1,2 (1SchoolofLifeScienceandEngineering,SouthwestUniversityofScienceandTechnology,Mianyang,Sichuan621010,China; 2FundamentalScienceonNuclearWastesandEnvironmentalSafetyLaboratory,SouthwestUniversityofScienceandTechnolo gy,Mianyang,Sichuan621010,China) 犃犫狊狋狉犪犮狋:Inthisstudy,therelativeexpressionof犖狋犕犜犘1wasdeterminedbyquantitativerealtimePCR (qRTPCR).Theresultsshowedthat犖狋犕犜犘1genewashighlyexpressedinflowersandleaves.After treatmentof400μmol/LZnSO4for48h,theexpressionof犖狋犕犜犘1increasedtothemaximum,which was3.81foldsofthecontrol.PlantexpressionvectorpBI12135S∶∶MTP1wasconstructed,andthere combinantplasmidwasintroducedinto犖犻犮狅狋犻犪狀犪狋犪犫犪犮狌犿L.cvNC89bymeansof犃犵狉狅犫犪犮狋犲狉犻狌犿狋狌犿犲 犳犪犮犻犲狀狊mediatedtransformation.TheqRTPCRrevealedadistinctincreaseofendogenous犖狋犕犜犘1ex pressionlevelintheoverexpressingtransgeniclines,whichwere10.42,7.61and11.84foldsofthatof wildplants,respectively.Seedsofwildtypeandtransgenicplantsweretreatedwithdifferentconcentra tionsofZnSO4.Theresultsshowedthatoverexpressionof犖狋犕犜犘1conferredenhancedzinctolerance. Theseresultsprovideanimportantbasistoiluminatethefunctionof犖狋犕犜犘1geneinZn2+transportin tobacco. 犓犲狔狑狅狉犱狊:犖犻犮狅狋犻犪狀犪狋犪犫犪犮狌犿;metaltoleranceprotein1;zinc;tolerance   ZnÒ#ÓԐÕzÖ×ØÙÚÛ,Ò#Ü ÝÉ300ÞßàF2000Þá¡â„Zã=„ Z , F#A6Ùäå

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