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Molecular Cloning and Expression Analysis of TaSF3B2 from Wheat

小麦胁迫相关基因TaSF3B2的克隆及表达特性分析



全 文 :书!"#:10004025(2016)08152806               犱狅犻:10.7606/j.issn.10004025.2016.08.1528
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|ŽžŸZTaSF3B2。,< %Jg¡¢,TaSF3B2£¤¥¦§l(犌犚犃犞犢)Z-0.895,¨ ©ª§lZ 43.92,«_¬­wQ”®=ed¯°i±3。²³´µª¶PCRJg·¸,犜犪犛犉3犅2*+_¨Œ¹Qº_» ¼·½ ,¨ ”1³¾¿·½Àº_»¼ ; _ÁÂÃÄQ , |*+·½ÅÆÇ 、 ÈÉ 、 vÊË 、 ÌÍÎABAÏÐbc ¸¡ÑÒ·½ , Ó_ÁÂÔQÕÖ¨¸¡ 。 ×Ø犜犪犛犉3犅2*+ÙÚ[\ÛÜvÝÑd,ޔ1,³_[\ß “`abcdfàQá–2Gd=e 。 9GH : [;犜犪犛犉3犅2;*+rs;,< %;*+·½
IJCK :Q785;Q786 !LMNO:A 犕狅犾犲犮狌犾犪狉犆犾狅狀犻狀犵犪狀犱犈狓狆狉犲狊狊犻狅狀犃狀犪犾狔狊犻狊 狅犳犜犪犛犉3犅2犳狉狅犿犠犺犲犪狋 HUANGXin1,XIABaicheng1,LONGXiangyu2 (1ColegesofAgronomy,HainanUniversity,Haikou570228,China;2RubberResearchInstitute,ChineseAcademyofTropical AgriculturalSciences,Danzhou,Hainan571737,China) 犃犫狊狋狉犪犮狋:Tostudythefunctionofsplicingfactorresponsestoabioticstress,acDNAsequenceof犜犪犛犉3犅2 wasisolatedandanalyzedfromwheatESTdatabase.Theresultsshowedthat犜犪犛犉3犅2containeda1854 bpORFencoding617aminoacids.UsingthemethodsofbioinformaticstopredictandanalyzeTaSF3B2, theproteinhasclassicdomainofsplicingfactor3Bsubunit2,highhydrophilia(GRAVY,-0.895)and heatstability(Instabilityindex,43.92).TheresultsofrealtimePCRrevealedthattheexpressionof 犜犪犛犉3犅2haddifferentlevelsindifferentdevelopmentsandtissues.Inaddition,theexpressionwasin ducedmarkedlybydrought,salt,lowtemperature,ABAandyelowrust(CY32),withdownregulation inleavesofseedling,butnochangeinroot.Insummary,itisspeculatedthatTaSF3B2couldplayarole notonlyingrowthanddevelopmentconditionsbutalsoinbioticandabioticstressesofwheat. 犓犲狔狑狅狉犱狊:wheat;犜犪犛犉3犅2;geneclone;bioinformaticanalysis;geneexpression   _âw,Q,ãäRNA(premRNA)åGæ
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XP_002519025.1)、+M, (犃狉犪犫犻犱狅狆狊犻狊犾狔狉犪狋犪
subsp.犾狔狉犪狋犪,XP_002867835.1)ñSF3B2(Spli
cingfactor3b,subunit2)Š*yz{d€…iJ*
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37.93%dŠ*y‹*ÙÚα:;(alphahelix),
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M.Marker;1.PCRÍÎ- ä1 犜犪犛犉3犅2*+cDNAdPCRÍÎÄÛ M.Marker;1.PCRproduct Fig.1 Electrophoresisof犜犪犛犉3犅2cDNAfragment 0351 ! " #  % &                   369
ä2 #SF3B2Š*yz{’“ Fig.2 AlignmentofaminoacidsequencesofSF3B2inplants Rá , Sá , TáñUáVW·α:;,ÒÓ<,β)>ñ8H9ò ä3 [\TaSF3B2Ž3dÿ7—˜Jg Theblue,red,greenandpurplelinesrepresentthealphahelix,extendedstrand,betaturnandrandomcoil,respectively Fig.3 SecondarystructureofwheatTaSF3B2protein * 、 ( 、¯ ¯ê 、 XY 、 3& 、 +M, 、 ZÎ[Q

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TaSF3B2Ú¥kk§6CdKLSF3B2dŽ z{€…iƽ83%,Ú¿¥kk§6ld+M, SF3B2dŽz{À–6Ædj÷i(69%)。ù z{’“Jg¡¢móg#SF3B2_Cüº_
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n¿_ GUS1—˜™,Ó¨/ SF3B2dNüŠ*yz{Jªº_¸¡»¼,Š* yz{dùGiÀFGº_› NüVªdo™。 fg 、 Š*y‘iÎz{’“—5·¸ ,SF3B2 ŽJK÷Öj“¨ú , «¿JK÷ÖÚ$/ªd ,$÷Ök§p›€… 。 13518¾           6 7,?:[\bcjk*+犜犪犛犉3犅2drsη½ViJg ä4 ÚTaSF3B2j÷dSF3B2Ž÷ÖJg Fig.4 PhylogeneticanalysisofSF3B2fromplants ä5 犜犪犛犉3犅2*+_¨³¾ÎŒ¹Qd·½Wõ Fig.5 Expressionpatternanalysisof犜犪犛犉3犅2in differentperiodsandtissues ä6 ¨bcÑ犜犪犛犉3犅2*+_¾ÔQd·½Wõ Fig.6 Expressionpatternanalysisof犜犪犛犉3犅2in rootunderstress 2.3 犜犪犛犉3犅2,:;rsAtB?@CD ‘e²³´µª¶PCRºùJg犜犪犛犉3犅2 *+_[\¨”1³¾ 、¨ Œ¹Qd·½Vi , _¾dÔ 、 ¬ÎÃK , ï¾dÔ 、 ¬ 、 ÃKÎ/K Q , ¤°ëØÿ犜犪犛犉3犅2*+d·½,·¸|*+ _[\d¨”1³¾d¨Œ¹Q¤·½ ( ä 5)。_[\d¾,犜犪犛犉3犅2*+_ÃQ·½¶± Æ , Ô}J , ¬Q·½¶±È ; _[\ï³¾ , 犜犪犛犉3犅2*+_ÔQ·½¶±Æ,/KQ}J,à KάQ·½¶±È 。 qýŒ¹V¼·½—5 , r ä7 ¨bcÑ犜犪犛犉3犅2*+_¾ÃÄQd·½Wõ Fig.7 Expressionpatternanalysisof犜犪犛犉3犅2in leafunderstress s犜犪犛犉3犅2*+_WëØdŒ¹Q¤·½,tö |*+uº_Œ¹V¼i·½ , ³|*+d·½ WõŨ”1³¾dvw 。 2.4 犜犪犛犉3犅2,:uvw67x;?@yzCD Z÷€%U犜犪犛犉3犅2*+dð°,Jg |*+_[\¾V/bcÑd·½Wõ , _Ç 、 ABAÎÌÍbcÑ,犜犪犛犉3犅2*+_ÔQ·½Õ Ö¨¸¡ , m¯°Ú|*+_[\¾ÔQd·½ ¶j“6Ȗk ( ä6);_[\¾dÃÄQ,|* +ÅÇ 、ABA、ÌÍ、ÈÉÎvÊËdŠ‹ÑÒ· ½ , «ÑÒ_l¤_6_.,¿QÈɊ‹|*+ ÑÒ±Z¸¡ , ¿}ZvÊË 、ABA、ÌÍÎÇbc ( ä7)。.d—5·¸,犜犪犛犉3犅2*+ÅÆÇ、 ÈÉ 、 ¤Ë 、 ÏÐÎÌÍ?bcdÒK , ×د°Ù Ú[\,Úû,bcßx 。 3 y Z RNA]^z{º_›âw,$Q,)/#d premRNAåæf]^ädçè#ïd mR 2351 ! " # $ % &                   369 NA,]^äŒ&ö€Æø–zd8|fà,]^ +K_-fàQ”®}2G=e , ~]^"ã") * , ì¡K¨.Î]^ã"dj€=e 。 9CD _[\bcjkdESTlmnQJSqrsuÿ €SF3Bœ*d€E,¿Š*yz{Jg¡¢| *+Z‚8$SF3B2ƒSAP145d‘*+。 _‚8$d]^äQ ,SAP145ÚSAP49#ü ýä ,VPr(HIV1d„š*+-$)¯ .;f̅ SAP145SAP49üýäd#Hӆ‡]^ädŒ & , ·¸SAP145_]^ädŒ&Q”®}2G= e , ×ØTaSF3B2_[\]^äŒ&Qá–j =e [812]。 _#Q

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]#÷‰‘h`ad,M’ | [1517]。 9CDJg]^+K犜犪犛犉3犅2*+_ ¨Œ¹ÎV/bcÑd·½Wõ , —5¡¢|* +_¨”1³¾º_Œ¹V¼i·½ , q«_Á ÂÃÄQd·½¶Åù/bcvw , ×ØTaSF3B2 ;f҈]^ädŒ&÷Ó_[\”1Î,Úû
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