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作 者 ：商佳胤1,李树海1,集 贤2,黄建全1,朱志强2,田淑芬1*

期 刊 ：西北植物学报 2012年 9期 页码：1829~1835

关键词：生物有机肥；贵人香葡萄；芳香化合物；

Keywords:bio-organic fertilizer, Italian Rieslings grape, aromatic compounds,

摘 要 ：于7月22日对5年生‘贵人香’葡萄分别用生物有机肥Ⅰ号和Ⅱ号进行0.02%根施和0.01%叶面喷施处理，待果实成熟后（9月22日），采用固相微萃取技术及气相-质谱联用技术测定葡萄果实的芳香物质含量，以探讨2种生物有机肥对‘贵人香’葡萄果实香气组分的影响。结果表明，（1）2种生物有机肥各处理的芳香化合物的检出种类分别为33、28、31、27种，均显著高于对照的22种，但对芳香化合物检出率的影响不显著。（2）使用生物有机肥对‘贵人香’葡萄果实中相对含量最多的4种芳香化合物2-己烯醛、己醛、2-己烯-1-醇、1-己醇的影响不显著，但可显著提高具有特殊香味的橙花醇、里那醇、松油醇、大马酮的相对含量，其中根施0.02%生物有机肥Ⅰ号与对照的差异最显著，橙花醇、里那醇、大马酮相对含量较对照分别提高了320.0%、485.7%、860.0%，松油醇的相对含量为0.63%，在对照中未检测出。（3）使用生物有机肥可以显著提高葡萄果实中酮、酯、酸、苯衍生物、烯烃类化合物相对含量，各处理均显著高于对照。

Abstract:In order to investigate the influence of two bio-organic fertilizers on aroma compounds,5 year old Italian Rieslings grape were treated with 0.02% root application and 0.01% foliage spraying,respectively.Solid phase micro extraction (SPME) and gas chromatography mass spectrometry (GC/MS) were used for determination after fruit mature.The results showed that:(1)The detection aroma compounds were 33,28,31 and 27 with 2 bio-organic fertilizers and 2 treatments,respectively.They are all higher than 22 kinds of the CK;but there were no significant effects of the detection rate on aromatic compounds.(2)Using bio-organic fertilizer had no significant effects on 2 kinds of aromatic compounds,which had the most relative content including 2-hexenal,hexanal,2-hexen-1-ol and 1-hexanol,but it could significantly increase the ralative content of nerol,linalool,terpineol and damascenone,which were related with the special aroma compounds.The root application with 0.02% of bio-organic fertilizerⅠwas the best;The relative content of nerol,linalool and damascenone were 320.0%,485.7%,860.0% higher than that of CK respectively;the relative content of terpineol was 0.63%,which was not detected in CK.(3)Using bio-organic fertilizers could significantly increase the relative content of ketone,ester,acid,benzene derivatives and alkenes compounds in grape berry,all treatments were higher than that of CK.

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