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作 者 ：于小萌1,2，徐碧玉2，刘菊华2，贾彩红2，张建斌2，王甲水3，金志强2,3*

期 刊 ：西北植物学报 2012年 7期 页码：1316~1321

关键词：香蕉；MaCAM；序列分析；荧光定量PCR；

Keywords:banana, MaCAM, sequence analysis, quantitative RT PCR,

摘 要 ：从香蕉根的cDNA文库中获得了一段香蕉钙调蛋白基因的片段，采用RACE技术获得其全长，命名为MaCAM。该基因全长845 bp，编码149个氨基酸。生物信息学分析表明，该蛋白属稳定蛋白，其等电点为4.12，有2个保守的EFh功能结构域。与已知植物的钙调蛋白基因相比，一致性达90%以上。其中与粳稻、油棕、胡萝卜、甘蔗的CAM编码的氨基酸序列的一致性分别为99.33%、96.71%、98.00%、98.66%。系统进化树比对分析显示，香蕉与甘蔗的亲缘关系最为密切。器官特异性分析表明，MaCAM在香蕉的根、球茎、叶片、花和果实中均有所表达，在根中表达量最高，花中次之，而在叶片中的表达量最低。

Abstract:A calmodulin gene named MaCAM was obtained by RACE technology based on a fragment from banana’s root cDNA library.The full length of this gene was 845 bp and encoded 149 amino acids.The result of bioinformatics showed that this protein is a stable protein with two conserved function domains——EFh,which pI is 4.12.Compared with other plant calmodulin genes,the identity of MaCAM was more than 90%.Amino acids identity analysis indicated that MaCAM had 99.33%,96.71%,98.00%,98.66% similarity compared with Oryza sativa var.japonica,Elaeis guineensis,Daucus carota and Saccharum officinarum,respectively.This gene had the most familiar genetic relationship with that of S.officinarum.RT PCR analysis showed that MaCAM was constitutively expressed in roots,stems,leaves,flowers and fruits.The expression level was the highest in root,flower followed in,and was the lowest in leaves.

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