作 者 ：刘志雄1,2，李来运1，李凤兰2

期 刊 ：西北植物学报 2015年 8期 页码：1506~1510

关键词：日本晚樱；花发育；MADS-box；PrseSHP；

Keywords:Prunus lannesiana, flower development, MADS-box, PrseSHP,

摘 要 ：采用同源克隆方法结合RACE技术，从日本晚樱（Prunus lannesiana）品种‘大岛樱’中克隆到花发育调控相关的PrseSHP基因（GenBank登录号为GU362645）。PrseSHP基因序列全长1 223 bp，包含1个长741 bp的完整开放阅读框，编码246个氨基酸和1个终止密码子。分子系统发生分析表明，PrseSHP属MADS-box转录因子的PLE/SHP进化系，并与蔷薇科植物的SHP同源蛋白聚于同一进化分支；蛋白序列比对显示，该转录因子拥有M、I、K和C共4个结构域，且其C末端结构域中包含高度保守的AGⅠ和Ⅱ基序。基因表达分析表明，PrseSHP基因主要在‘大岛樱’的花瓣、雄蕊、雌蕊和幼果等器官中表达，在花萼中仅能检测到微弱的转录信号，在幼叶中不表达，与其他植物SHP同源基因的表达模式有一定的差别。功能分析显示，转PrseSHP基因拟南芥植株明显比野生型拟南芥弱小，转基因拟南芥在6～8片莲座叶后即抽薹开花，时间较野生型拟南芥（14～17片莲座叶后抽薹开花）明显提前，证明异位表达的PrseSHP基因能促进拟南芥早花，其在花发育过程中可能参与调控植物开花。

Abstract:Full cDNA of one MADS-box gene,PrseSHP with GenBank accession No.GU362645,was cloned from Prunus lannesiana using homologous cloning and RACE method.The full length of PrseSHP cDNA is 1 223 bp，containing an open reading frame（ORF）of 741 bp and coding for a polypeptide of 246 amino acid residues.Sequence and phylogenetic analyses grouped PrseSHP into PLE/SHP lineages of the MADS-box family.Conceptual translation revealed that PrseSHP contain MADS,I,K and C domains.Expression analysis suggested that PrseSHP expressed mainly in petal,stamen,gynoecium and young fruit of P.lannesiana ‘Makino’.Moreover,functional analysis suggested that transgenic Arabidopsis was obviously dwarf,and flowering during 6-8 rosette leaves,which was early than that of wild-type Arabidopsis who was flowering during 14-17 rosette leaves.Ectopic expression of PrseSHP could obviously promote flowering of transgenic Arabidopsis.Our results suggest that PrseSHP are involved in flowering in Cherry Blossom.