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作 者 ：袁金玲，郭广平，岳晋军，吴晓丽，顾小平 *

期 刊 ：西北植物学报 2012年 1期 页码：60~66

关键词：孝顺竹；开花过程；DNA甲基化；

Keywords:Bambusa multiplex, flowering process, DNA methylation,

摘 要 ：以孝顺竹为材料，利用HPLC和MSAP技术在其未开花至开花的生长过程中进行甲基化水平的检测，分析竹子开花过程中基因组DNA甲基化的动态，以揭示DNA甲基化水平与竹子开花现象的相关性。结果显示：（1）孝顺竹基因组DNA甲基化率在不同的时间处于动态变化中，进入开花状态的孝顺竹植株其甲基化水平极显著低于开花竹丛中未开花植株和未开花竹丛；开花和未开花植株的总甲基化率分别为9.00%和12.42%，全甲基化率分别为5.06%和7.53%。（2）MSAP位点中有66.83%的位点在开花和未开花材料中甲基化状态保持一致，33.17％的位点在开花和未开花植株中发生甲基化变化：其中22.28％的位点在开花植株中发生完全的去甲基化，1.98%的谱带在开花植株中发生甲基化，8.91％的位点在开花和未开花材料中甲基化水平呈现上升或降低的趋势。研究表明，开花的孝顺竹同时发生甲基化和去甲基化，但发生去甲基化的概率明显大于发生甲基化的概率，最终导致其甲基化水平极显著降低。

Abstract:To understand the DNA methylation features of bamboo during its flowering process,we employed the techniques of HPLC (high performance liquid chromatography) and MSAP (methylation-sensitive amplified polymorphism) to detect the DNA methylation level of Bambusa multiplex (Gramineae,Bambusoideae),in a continuous period from its non-flowering to flowering stage.The results showed that DNA methylation rate of B.multiplex changed dynamically at different times.Furthermore,DNA methylation rates of flowering plant were significantly lower than those of non-flowering plant and non-flowering clumps.Total DNA methylation rates of 9.00% and 12.42% were detected in flowering and non-flowering plant,respectively,with full-methylation of 5.06% and 7.53%.Meanwhile,66.83% MSAP sites were identical both in flowering and non-flowering plants and 33.17% sites varied.Among those 33.17% variable sites,22.28% were demethylation and 1.98% were methylation in flowering plants,and other 8.91% variable sites gave a variance tendency between flowering and non-flowering plants.Therefore,demethylation and methylation happened simultaneously in flowering plants,with a much higher demethylation rate than that of methylation,thus exhibited a low methylation rate in flowering plants.

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