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作 者 ：袁金玲;岳晋军;吴晓丽;张朵;郭广平;顾小平*

期 刊 ：植物研究 2010年 30卷 5期 页码：562-567

关键词：孝顺竹；愈伤组织；增殖；培养基；

Keywords:Bambusa multiplex, callus, proliferation, medium, optimization,

摘 要 ：为筛选适宜孝顺竹愈伤组织继代增殖培养基，控制褐变发生，提高再生体系效率，对培养基组成如5种基本培养基、6种有机添加物、7种糖类和5种大量元素等因子进行试验分析。结果表明：培养20 d，基本培养基以MS效果较好，愈伤组织增殖2.8倍，白至淡黄色，致密；有机添加物以1.0 g·L^-1脯氨酸效果较显著，愈伤组织增殖3.64倍，淡黄色，致密均一；碳源以30 g·L^-1麦芽糖效果较好，愈伤组织增殖2.96倍，白至淡黄色，致密。5种大量元素中NH₄NO₃对孝顺竹愈伤组织增殖的影响达到显著水平，以825 mg·L^-1为较佳浓度，培养29 d愈伤组织增殖可达5倍以上，部分出现根分化；适宜孝顺竹愈伤组织培养的大量元素组合为：KNO₃ 475 mg·L^-1+NH₄NO₃ 825 mg·L^-1+MgSO₄·7H₂O 185 mg·L^-1+KH₂PO₄ 340 mg·L^-1+CaCl₂·7H₂O 440 mg·L^-1。

Abstract:An effective regeneration system is the pre-requisite for genetic transformation of bamboos. Medium components for callus proliferation of Bambusa multiplex were studied, 5 basal medium, 6 organic additives, 7 sugars, and 5 macronutrients were compared. The results showed that Murashige and Skoog (MS) basal medium was effective, callus increased 2.8 times at 20 days, white to yellowish, compact; 1.0 g·L^-1 proline was suitable the organic additive, callus increased 3.64 times, yellowish, compact and even; 30 g·L^-1 maltose was the optimal carbon source, callus increased 2.96 times, white to yellowish, even. Among five macronutrients, NH₄NO₃ has significant influence on callus proliferation (α＝0.05) and its optimal concentration was 825 mg·L^-1, callus could increase over 5 times at 29 days, with some rooting; KNO₃ 475 mg·L^-1+NH₄NO₃ 825 mg·L^-1+MgSO₄·7H₂O 185 mg·L^-1+KH₂PO₄ 340 mg·L^-1+CaCl₂·7H₂O 440 mg·L^-1 is a suitable combination for callus proliferation.

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