---

作 者 ：董 娜,张军刚,胡铁柱,陈向东,张亚娟,茹振钢

期 刊 ：植物病理学报 2016年 46卷 3期 页码：384-393

关键词：小麦；白粉病；表达谱芯片；信号通路；防卫反应；

Keywords:wheat, powdery mildew, microarray, signal transduction pathway, defense response,

摘 要 ：Edinburgh-b是从英国爱丁堡引进的小麦抗白粉病新种质，为了深入了解该种质的抗白粉病分子机制，本研究采用Agilent公司的4 SymboltB@ 44 k小麦表达谱芯片，以Edinburgh-b接种白粉菌0 h为对照，分析其在白粉菌胁迫下的基因表达谱。结果表明，在43 603个探针中筛选出差异表达探针5 835个，其中上调表达2 801个，下调表达3 034个。差异表达基因主要包括与代谢相关的酶类、转录因子、病程相关蛋白、防卫反应基因和信号传导因子等。Pathway分析显示苯丙烷类生物合成、水杨酸信号通路、茉莉酸生物合成、活性氧代谢等被激活，乙烯生物合成和生长素信号通路被抑制，推测水杨酸和茉莉酸信号通路共同参与了Edinburgh-b对白粉菌的防御反应。选取上调和下调表达共17个基因进行实时定量RT-PCR验证，表明基因芯片数据具有良好的重复性。

Abstract:Wheat germplasm Edinburgh-b, introduced from Edinburgh, United Kingdom, displays immune to 33 current popular isolates of Blumeria graminis f. sp. tritici in China. To elucidate the resistance molecular mechanism of Edinburgh-b to powdery mildew, its gene expression profiles under infection compared with that at 0 hour after inoculation were analyzed by using the Agilent 4 SymboltB@ 44 k wheat microarray. There were 5 835 altered expression probes among total 43 603 probe sets in the microarray plate, including 2 801 up-regulated and 3 034 down-regulated ones. The differentially expressed genes with known functions included enzymes involved in metabolism processes, transcription factors, pathogenesis-related proteins, defense response, and signal transduction. The pathway analysis showed that some genes involved in phenypropanoid biosynthesis, salicylic acid signal transduction, jasmonic acid biosynthesis, and active oxygen metabolism were up-regulated, and some genes involved in ethylene biosynthesis and auxin signaling pathway were down-regulated. These results suggested that salicylic acid and jasmonic acid signaling pathways might be involved in the defense responses of Edinburgh-b to powdery mildew. Furthermore, the gene expression levels of 17 up- or down-regulated genes validated by quantitative real-time RT-PCR were accord with those by the microarray analysis.

---