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作 者 ：董邵云，苗 晗，张圣平，刘苗苗，王 烨，顾兴芳*

期 刊 ：西北植物学报 2012年 11期 页码：2177~2181

关键词：黄瓜；白色果皮；遗传分析；SSR标记；基因定位；

Keywords:cucumber, white fruit skin, genetic analysis, SSR marker, gene mapping,

摘 要 ：以黄瓜嫩果深绿色果皮自交系1507（P₁）和白色果皮自交系1508（P₂）为亲本，构建6世代遗传群体（P₁、P₂、F₁、F₂、BC₁P₁、BC₁P₂），对黄瓜嫩果白色果皮基因(w)进行遗传规律分析和基因定位研究。结果表明，黄瓜白色果皮性状由隐性单基因(w)控制，深绿色对白色为显性。利用F₂群体，结合分离群体分组分析法筛选得到了14个与w基因相关的SSR标记，构建了该基因的SSR连锁群，将其定位到黄瓜3号染色体上，两侧的标记为SSR23517和SSR23141，遗传距离分别为4.9 cM和1.9 cM。侧翼标记之间的物理距离为1 150 kb，在该区域中共预测了500个候选基因。该研究对w基因的初步定位，为该基因精细定位及分子标记辅助选择育种奠定了良好的基础。

Abstract:Inbred line 1507（dark green fruit skin） and inbred line 1508（white fruit skin） were used as the experiment materials for genetic analysis and gene mapping of white fruit skin in cucumber in this study.Genetic analysis showed that a single recessive nuclear gene,w,dominates the white fruit skin trait in cucumber.Bulked segregate analysis (BSA) and simple sequence repeat (SSR) technologies were employed to map w gene in F₂ population.w was mapped to a linkage group with 14 SSR markers,corresponding to chromosome 3 of cucumber.The flanking markers SSR23517 and SSR23141 were linked to the w gene with genetic distances of 4.9 and 1.9 cM,respectively.The physical distance between SSR23517 and SSR23141 was 1 150 kb based on the whole genome sequence of cucumber,and there are 500 candidate genes in this region.These results will benefit fine-mapping of w gene and marker-assisted selection (MAS) in fruit skin color cucumber breeding program.

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