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作 者 ：葛文雅1,2,4，惠 伟1*，闫洪波2,3,4，程玉豆2,4，关军锋2,4

期 刊 ：西北植物学报 2012年 5期 页码：871~875

关键词：鸭梨；羟基肉桂酰CoA莽草酸/奎宁酸羟基肉桂酰转移酶；酰基转移酶；基因克隆；绿原酸；

Keywords:Yali, HCT, acyltransferase, gene cloning, chlorogenic acid,

摘 要 ：羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶(HCT)是植物绿原酸合成的关键酶之一。该研究以鸭梨为材料，采用同源基因克隆的方法，克隆出一个鸭梨的HCT基因，命名为PbHCT3。PbHCT3基因cDNA全长序列为1 731 bp，基因编码序列长度为1 317 bp,编码438个氨基酸，含有酰基转移酶的2个保守序列HHAAD和DFGWG及保守结构域MVVNVTVRES。实时荧光定量PCR分析表明：PbHCT3基因在幼果果皮、果肉、果心、幼叶及花蕾期花瓣中的表达量较高。随着鸭梨果实生长，果实中PbHCT3基因表达量逐渐降低。研究表明，PbHCT3基因与鸭梨幼嫩组织的生长发育有密切关系。

Abstract:Hydroxycinnamoyl-coenzyme A:shikimate/quinate hydroxycinnamoyltransferase (HCT) is one of the key enzymes in plant chlorogenic acid synthesis.In this study,we isolated HCT gene by homologous cloning method from ‘Yali’,and named as PbHCT3.The full length of PbHCT3 cDNA is 1 731 bp,and the 1 317 bp open reading frame encodes 438 amino acids which containing two conservative sequences HHXXD and DFGWG and conservative structure MVVNVTVRES domain of acyltransferase.Real time PCR analysis indicated that PbHCT3 showed a higher expression in peel,pulp and core of young fruit,young leaves,and alabastrum,but the expression in fruit decreased with the fruit growth.These results suggested the PbHCT3 is closely related to growth and development of younger tissue in ‘Yali’.

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