Abstract:In order to investigate dynamics of Pst pathogenicity and stripe rust resistance of new wheat cultivars (lines), and provide basis for disease epidemiology, control and wheat breeding, a total of 2 779 stripe rust samples collected from 28 counties of 8 cities in Shaanxi province, and some areas of Gansu, Sichuan and Hubei provinces adjoining to Shaanxi province during 2002-2014 were identified on 19 differential hosts. In total, 45 races and pathotypes were detected. Among these, CYR33 and CYR32 can be detected each year and are the most prevalent races in Shaanxi province. Although the occurrences frequencies of the new pathotypes G22-9 and G22-14 are low now, they are virulence to Guinong, 92R and Moro derivatives, and the frequencies are increa-sing. The Hybrid46 group and Shuiyuan11 group were the dominant groups, the total frequency of the two groups was kept stable about 80%, which was closely related to the low diversities of resistant genes in Chinese wheat population. Therefore, the new and different kinds of stripe rust resistance materials should be developed and used in wheat breeding programs. In addition, 2952 new bred wheat cultivars (lines) were evaluated by prevalent Pst races during 2002-2014, the results indicated that the resistance level has been increasing in recent years. According to the results of Pst races detection and wheat resistance analysis, the current prevalent Pst races CYR32, CYR33 and new pathotypes G22-9, G22-14 should be paid more attention in wheat breeding programs.