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作 者 ：余朝阁1,2，魏 岚3，李天来1*，刘志恒2，李琳琳1，张亢亢1

期 刊 ：西北植物学报 2012年 6期 页码：1166~1170

关键词：番茄；灰霉病；钙；水杨酸；防御酶活性；

Keywords:tomato, gray mold, calcium, salicylic acid, defensive enzymes,

摘 要 ：以番茄‘L402’品种幼苗为试材，经水杨酸（SA）诱导处理后接种灰霉病菌，再进行外源Ca²⁺、Ca²⁺螯合剂和Ca²⁺抑制剂处理，分析Ca²⁺和SA处理番茄叶片对灰霉病抗性和主要防御酶系活性的变化，探讨Ca²⁺和SA对番茄诱导抗病性的影响。结果显示：（1）外源SA可显著提高番茄诱导叶和非诱导叶抗灰霉病能力，²⁺能进一步增强SA诱导的抗病能力；而Ca²⁺螯合剂EGTA和质膜钙通道抑制剂LaCl₃则不同程度地抑制了SA诱导的番茄灰霉病抗性。（2）外源SA能提高番茄诱导叶和非诱导叶中苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、过氧化物酶（POD）活性，外源Ca²⁺亦进一步增强了SA诱导的上述防御酶活性，但缺钙处理则不同程度降低这些防御酶活性。（3）外源补充Ca²⁺及不同缺钙处理对SA诱导的过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性未发现规律性影响。研究表明，钙对SA诱导番茄抗灰霉病性的增强效应，可能与其提高SA诱导番茄叶片中PAL、PPO和POD等防御酶活性有关。

Abstract:Tomato cv.L402 was used as plant material.Resistance to gray mold and activities of defensive enzymes were investigated after sprayed Ca²⁺ or its antagonist on tomato.The results showed that:(1)Resistance to gray mold were improved significantly for induced and un induced leaves in tomato treated with SA.Induced resistance was farther enhanced by Ca²⁺ but restrained badly by chelator as EGTA or the plasmalemma Ca²⁺ channel blocker as LaCl₃.(2)Activities of PAL,PPO and POD in induced and un induced leaves also rose after treated the plant with SA.This response was promoted by exterior Ca²⁺ and suppressed by the inhibitor of Ca²⁺ such as EGTA and La³⁺ in a general way.(3)Activities of CAT and SOD induced by SA were not regulated by Ca²⁺ or inhibitor of Ca²⁺.Consequently,modulating of calcium on the induced resistance to tomato gray mold by SA may has relations with the activities of defensive enzymes such as PAL,PPO and POD in tomato leaves.

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