全 文 ：书西北植物学报!
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基金项目$国家自然科学基金"
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作者简介$袁军文"
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基因的克隆及分析
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要$抱茎独行菜"
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#为十字花科具典型粘液繁殖体植物!为探究该植物中种皮粘液质基因
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!该基因在拟南芥中编码
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鼠李糖合成酶#的功能!通过生物信息学分析设
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基因!命名为
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&同源比对分析结果表明!
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与拟南芥
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结果表明!该基因在抱茎独行菜各组织中均有表达!在角果和根中的表达量最高!
且其表达量随角果的发育表现出渐强的趋势&免疫组织化学定位分析表明!
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基因于角果发育的早期阶段在
内珠被和外珠被都有表达!而在外珠被的表皮和亚表皮中表达量更高!至角果发育的最后阶段!其表达集中于表皮和
亚表皮层!这可能与抱茎独行菜的外珠被发育成种皮及粘液质的生成有关&将
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基因转化拟南芥!该基因的
过表达对位于粘液质合成途径中的上游基因
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具有显著的抑制作用&表型比对观察显示!转基因拟南芥与其
野生型植株形态无显著差异!这可能是因为抱茎独行菜种皮的发育和粘液质的形成是一个多基因调控的复杂过程!
某一基因的过表达或许不会引起明显的表型变化&
关键词$抱茎独行菜%粘液质合成%免疫定位%
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基因%
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