---

作 者 ：齐飞艳，胡 陶，彭镇华，高 健*

期 刊 ：西北植物学报 2013年 1期 页码：48~52

关键词：内参基因；PheTFL1；表达；实时荧光定量PCR；

Keywords:reference genes, PheTFL1 gene, expression, qRT-PCR,

摘 要 ：通过qRT-PCR对毛竹相关成花基因PheTFL1的表达进行研究，为毛竹开花机理的研究提供理论依据。从毛竹UBC18、PP2A和EF1α等9个候选内参基因中筛选出在叶、幼嫩花序、花序轴、枝、竹青等11个组织器官中都稳定表达的PP2A用于毛竹PheTFL1基因qRT-PCR结果的校正。结果显示：PheTFL1基因在开花竹叶、枝和竹青中低丰度表达，与未开花竹差异不显著，但在花和花序轴中高丰度表达；在实生苗叶和根中高丰度表达，在实生苗茎中低丰度表达。PheTFL1基因在具有分生能力的幼嫩组织中高丰度表达，说明其不仅参与花发育的调控，还参与了分生组织生长的调控。

Abstract:In this study the expression analysis of PheTFL1 gene related to moso bamboo flowering provide the theory basis for the research of flowering mechanism of moso bamboo.PP2A gene was screened from nine candidate reference genes including UBC18,PP2A and EF1α,and so on,because of its stable expression in 11 kinds of different tissues and organs such as the leaves,immature inflorescence,inflorescence axis,branches and the cuticle of bamboo stem,and so on,which is used to correct the qRT-PCR results of PheTFL1 gene.The results showed that the low level expression of the PheTFL1 in leaves,branches and cuticle of bamboo stem of flowering bamboo was not significantly different from those of non-flowering bamboo,while there was high level expression in immature inflorescence and inflorescence axis.The expressions of PheTFL1 were detected at high level in leaves and roots of seedlings,but at lower expression in stems.Appreantly the PheTFL1 gene were abundantly expressed in young tissues which had meristematic ability,it may be involved in regulating the growth of the meristems,as well as in the development of flower.

---