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作 者 ：梁东  马锋旺  张敏

期 刊 ：西北植物学报 2008年 1期

关键词：苹果；山梨醇；6-磷酸山梨醇脱氢酶；原核表达；

Keywords:apple, sorbitol, sorbitol-6-phosphate dehydrogenase, prokaryotic expression,

摘 要 ：6-磷酸山梨醇脱氢酶(sorbitol-6-phosphate dehydrogenase,S6PDH)是蔷薇科植物中合成山梨醇的重要酶。以苹果叶片为材料,利用RT-PCR法克隆到S6PDHcDNA全长,将其与大肠杆菌表达载体pET-32a( )构建原核表达载体pET-S并转化大肠杆菌BL21,经IPTG诱导表达后,SDS-PAGE检测结果表明该基因表达了1个约54kD的蛋白,为进一步研究目的蛋白的结构和功能提供了试验基础。

Abstract:Sorbitol-6-phosphate dehydrogenase(S6PDH) is a key enzyme for the biosynthesis of sorbitol in Rosaceae plants.S6PDH cDNA was amplified by RT-PCR from apple leaf,then was cloned into pET-32a( ) vector to construct recombinant prokaryotic expression vector

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