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作 者 ：陈惠萍  徐朗莱

期 刊 ：西北植物学报 2006年 3期

关键词：壳聚糖；不结球白菜；

Keywords:GDHmRNA, GSmRNA, RT-PCR,

摘 要 ：应用半定量RT—PCR技术检测了壳聚糖对不结球白菜叶片GDH和GS的mRNA水平的影响。结果表明GDHmRNA的变化与GDH活性的变化趋势基本一致，而GSmRNA的量基本恒定，并未与GS的活性变化相符合。即壳聚糖可能在转录水平上对氨同化关键酶GDH进行调控；而对GS的调控则不在转录水平上，有可能发生在翻译水平上。

Abstract:Semi-quantitative RT-PCR was adopted to determine the effects of chitosan on the levels of GDHmRNA AND GSmRNA in the leaves of no-heading Chinese cabbage, and the results showed that GDHmRNA tended to vary basically in the same pattern as GDH activity did

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