以自交亲和的苹果品种早红香和自交不亲和的苹果品种斗南、富士、金冠为试验材料,对授粉后花粉管生长情况,花柱SOD,POD,CAT活性,MDA,蛋白质含量及4种内源激素含量进行研究。结果表明: 自交不亲和的品种异花授粉和自交亲和的品种自花授粉后花粉管能够正常生长并通过花柱基部。自交不亲和的品种自花授粉后0~24 h花粉管能正常生长,当花粉管生长到距柱头1/2时停止生长; 自交亲和的苹果品种早红香自花授粉后0~48 h花柱SOD,POD,CAT活性相对稳定,自交不亲和品种自花授粉后24~72 h(花粉管已停止生长)3种酶活性及MDA含量变化较大; 自交亲和的苹果品种早红香自花授粉后24 h花柱GA3含量达到峰值而ABA含量下降,而自交不亲和的品种自花授粉后12~48 h花柱ABA含量开始大幅上升,自交亲和品种自花授粉后12~24 h(IAA+GA3)/ABA值急剧上升,之后开始下降,但(IAA+GA3)/ABA值高于其他自交不亲和品种。
Growth of pollen tube and style physiology characteristic index after pollination were studied for Self-compatible apple cultivar ‘Zaohongxiang‘ and self-incompatible apple cultivars ‘Dounan‘, ‘Fuji‘, ‘Golden Delicious‘. The results showed that: pollen tube grew normally and entered into the style base after self-pollination of self-compatible cultivar and cross-pollination of self-incompatible cultivar. Self-pollination pollen tube of self-incompatible cultivars grew normally after self-pollination within 24 h and stopped growing after 48~72 h when the pollen tube reached the 1/2 of the style to stigma. The style SOD, POD and CAT activities of self-compatible cultivar ‘Zaohongxiang‘ were relatively stable within 48 h after self-pollination. The SOD, POD, CAT activities and MDA content of self-incompatible cultivars had great changes after self-pollination 24~72 h when the pollen tube stopped growing. Style GA3 content of self-compatible cultivar ‘Zaohongxiang‘ had a peak and the ABA decreased in 24 h after self-pollination. Style ABA content of self-incompatible cultivars increased significantly after self-pollination 12~48 h. The(IAA+GA3)/ABA ratio sharply increased at 12~24 h after self-pollination and then decreased in ‘Zaohongxiang‘, but it still was higher than that of self-in compatible cultivars.
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