从河南濮阳、山东菏泽等地的欧美杨溃疡病发病林地采集病样96份，经分离、纯化、归类出10种分离物，其中镰孢属（Fusarium）真菌占优势。以离体苗干水培接种和盆栽苗接种，经致病性测定，明确分离物Mz01为致病菌株。经形态学鉴定，Mz01为茄镰孢菌(Fusarium solani)。采用真菌通用引物对分离物Mz01进行PCR扩增，对其核糖体DNA(rDNA)内转录间隔区测序，并将测序结果在GenBank中进行同源性比对分析，分子鉴定与形态学鉴定一致。茄镰孢菌作为我国欧美杨溃疡病的病原菌系首次报道。
A new canker disease occurred on Populus ×euramericana cv‘74/76’ and P.×euramerican ‘zhonglin 46’in Puyang，Henan Province and Heze，Shandong Province in resent years，and caused severe damage to the poplars. Disease incidence increased year after year. Determination of the pathogen of the disease is in an urgent need. Ninetysix samples of the canker were collected from stands of diseased poplars and 10 isolates were obtained，among which most isolates belonged to genus Fusarium. The isolates were inoculated on both hydroponic stem and potted two years old seedlings of Populus ×euramericana cv ‘74/76’ for 4～5 days at 25 ℃. Reisolation from the lesion formed by the inoculation was the same as the inocula，demonstrating that the isolate Mz01 was a pathogenic strain of Fusarium solani based on its morphological. The sequences of the isolates Mz01 were amplyfied by PCR technique with universal primers of fungi，and the sequences were further compared with that in GenBank. The molecular identification confirmed the same results from morphology identification. This is the first report that Fusarium solani is the pathogen of canker disease in Populus ×euramericana in China.
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