健康的锥栗(Castanea henryi)皮层中,苯丙氨酸解氨酶(PAL)比活力与抗感病性之间不相关。不同抗病性的锥栗品种接种栗疫菌(Cryphonectria parasitica)后,PAL比活力均上升,接种144h后,PAL比活力抗病性强的品种比抗病性弱的品种高。接种前以及接种后的各个时间内,木质素含量都是抗病品种比感病品种高。绿原酸的含量与品种的抗病性之间不呈规律性的相关,从绿原酸对栗疫菌菌丝生长影响的测定结果可知,锥栗体内的绿原酸浓度不足以影响栗疫菌。可见,接种后的皮层PAL比活力以及接种前、后的皮层木质素含量可作为衡量锥栗抗病性强弱的指标。
The phenylalanine ammonialyase (PAL) speciaific activity in the healthy bark of Castanea henryi were not relative to the variety resistance, the PAL specific activity all increased in the different resistant varieties after inoculation, at 144hr the PAL specific activity in the resistant varieties were higher than that in the susceptible ones after inoculation. The contents of lignin in the resistant varieties were all higher than that in the susceptible ones before and after inoculation. The contents of chlorogenic acid in Castanea henryi were not relative to the resistance to the blight. So, the PAL specific activity after inoculation in bark could be used as the criterion to evaluate the disease resistance.
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