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作 者 ：金得森

期 刊 ：林业科学 1991年 27卷 2期 页码：132-139

关键词：白僵菌；载体；砖式半固体；

Keywords:Beauveria bassiana, Carrier, Brick type semi-solid,

摘 要 ：本文对利用载体进行砖式半固体开放培养法机械化生产白僵菌孢子粉和液体深层发酵的工艺中有关技术问题作了多方面试验研究,结果表明:1%麦皮+1.5%黄豆粉+0.5%玉米粉和1%麦皮+1.5%黄豆粉+1%玉米粉是理想液体培养基配方,平均产孢量分别达9.16亿/ml和8.51亿/ml,比土法生产常用5%麦皮浸出液提高1.65—1.85倍。筛选出C号半固体发酵培养基配方,平均产孢量达890.7亿/g,比常规土法生产提高7.91倍;选择出半固体发酵培养的理想载体R质粒;筛选出培养基、载体、接种量优化组合配比为1:3:6;载体平铺厚度以6cm为宜,其产孢量为125.7亿/cm~2,比4cm、2cm厚的分别提高0.86和3.1倍;不同菌龄菌液接种与固体发酵培养产孢量关系,以菌丝状态接种(含孢量806.2亿/g)比节孢子状态接种(含孢量573.1亿/g)提高41%;半固体发酵以13—15天为宜。

Abstract:In order to improve the existing method of Beauveria bassiana Prodution, We made many-sided and explorative experimental researches on the relevant technical problems in the technology of brick type semi-solid fermentation culture method to mechanizedly produce Beauveria bassiana spore powder and deep-level liquid fermentation by means of carrier and achieved some success. The experimental results suggested:FL-85055 and FL-86004 were ideal prescriptions of liquid culture medium and the average spore output was 9.16 hundred million/ml and 8.15 hundred million/ml respectively,which was 1.85 times more than the production by the indigenous method with common 5% wheat skin soak; screened out Number C prescription of semi-solid fermentation culture medium and the average spore output attained 890.7 hundred million/g,which was 7.91 times more than the fungicide by the regular indigenous method with spore output 100 hundred million/g; selected out the ideal carrier FLT-8504 cultured by the semi-solid fermentation; the ratio of optimization combination of the culture medium, carrier and inoculation quantity was 1:3:6.

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