Abstract:DNAs isolated from poplar trees I-69(),I-63()and two F1 clones named 34-17 and 34-135 were used as template to detect the polymorphisms by RAPD analysis. Of 200 primers, three primers amplified polymorphic products, and only one of them, OPAD-01 is linked likely to insectresist gene.RAPD analysis of 34-215 (suspective to pests) and 34-188(resistance to pests)also confirmed the results.