Abstract:This is a report on the experimental results of anther culture for genus Populus. Pollens of 14 spp (Populus simonii×P. nigra var. italica + Salix matsudana, P.simonii ×P. nigra, P. nigra var. italica, P. euphratica, P. pekineca, P. euramericana ev.‘Polska-15A‘ etc.) and nine were ased. Callus of anther was induced by BN or nitsel medium in which kinetin or auxin of know concentrotion was added. Differentiation was saccessful on modified MS medium (by adding BA, IAA or NAA of different concentration) and 900 plantlets were roised and tramplanted in seed bed or pots. Some of them reached a height of 3.9 m in three years. Chromosome counting of root tip cells showed that most of them are haploids. It was certain that the plantlets were of pollen origin.