Abstract:Coat protein gene segment of Strawberry mild yellow edge virus ( SMYEV) was amplified with some special primers by reverse transcription-polymerase chain reaction (RT-PCR) from strawberries plants,and testified by sequencing. A stable system for detection of SMYEV with RT-PCR was established, and among nineteen strawberries cultivars and two wild strawberries species checked, twelve cultivars and Fragaria pentaphylla were infected. The 932 bp segment in 3‘ terminal region of SMYEV genome was amplified from Shenyang isolate SY01. Comparison showed it shared 86%～96% nucleotide acid identity with the published sequences. Phylogenetic analysis shows that all isolates of SMYEV fell into three clades. The Shenyang isolate SY01 lay in the same clade with the Europe and America isolates, but formed a small separate branch. Analysis of RNA secondary structure showed that three stem-loop structures were formed in 3‘terminal region, and sequence differences in all isolates impacted little on the stem-loop structures.