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作 者 ：李成琼;周庆红;宋洪元;任雪松;司　军;吴能表

期 刊 ：园艺学报 2005年 32卷 5期 页码：878-880

关键词：甘蓝；槲皮素；自交不亲和性；SRK；

Keywords:Brassica oleracea L., Quercetin, Self-incompatibility, SRK,

摘 要 ：对甘蓝自交不亲和系‘022E1’, ‘022A1’, ‘02259’开花前两周的花蕾进行不同浓度的槲皮素处理, 开花后自交。与花期对照相比, 3个自交不亲和系的亲和指数差异达到显著性水平, 其中以1 500 μmol·L ^{- 1}槲皮素溶液处理效果最佳, 在022E1系中其亲和指数由原来的0.03增至4.35, 完全转化为了自交亲和系。采集开花前1 d的花蕾切取柱头进行SRK活性测定, 结果表明, 槲皮素处理后可显著抑制甘蓝自交不亲和系柱头中SRK活性, 从而阻止了自交不亲和性的信号传导, 达到了克服甘蓝自交不亲和性的目的。

Abstract:In this experiment, the Self-incompatible ( SI) lines budswere treated with different concentrations of quercetin solution (500, 1 000 and 1 500μmol·L ^-1 ). The results showed quercetin could overcome the SI of Brassica oleracea L. significantly. What‘smore, the most seeds per pollinated flower were obtained with the treatment of 1 500 μmol·L ^{- 1} quercetin solution among three concentrations. In addition, the self-compatible ( SC) index of 022E1 SI line increased from 0.03 to 4.35, which similarly to bud pollination. Quercetin acted as a kind of Srecep tor kinase ( SRK) inhibitor, it can p revent the signal conduction of SI since it reduced SRK activity by spraying certain concentration quercetin. So it is feasible to overcome the SI by spraying quercetin.

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