Abstract:We obtained core sequence of MAO3 of banana according to homo-cloning, and then cloned 5‘up stream and 3‘down stream sequences by RACE PCR technology and the promoter sequence by GenomeWalker technology. Phylogenetic analysis showed that this gene was highly conserved in the same species and had comparatively high homology compared with both monocots and dicots. In situ hybridization experiment showed that the expression of MAO3 gene was tissue specific. Meanwhile, according to fluorescence real-time quantification PCR, we found that MAO3 and ERS2 (one of banana ethylene receptor genes) genes were also induced by wounding.