全 文 :!23" !5#
Vol.23 No.5
! " # $
ACTA AGRESTIA SINICA
2015$ 9%
Sep. 2015
犱狅犻:10.11733/j.issn.10070435.2015.05.020
ûmü;ýþÿ÷Yûm*t|63
) Z1,[>1,\$|1,] 2^,SJ_2, ñ2
(1.J3*+(,+0 ÙnÝìæNñ:2¤ðC:=Vº,J3 3°810016;
2.78()*+N)+0,78 2730070)
45
:
GHkrìBkrX
,
Ikrìz²sBYy=pë8krâçB@g
。
*¥PKOVr
n\{âK§® 2u¡B(犕犲犱犻犮犪犵狅狊犪狋犻狏犪)、êÙ©(犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊)=aÙ©(犜狉犻犳狅犾犻狌犿狆狉犪狋
犲狀狊犲)¼ë
。
lmPJ
,
1ìkrëDor
(犘<0.01),irñë106.53~373.44mg·L-1。Uìz²
sBYy¾DNs
、
Hjs
、
dms
、
ws
、
+s
、
efs=ñÛs!
,
wz²sBYy=pëDo*
。
krìBkrë8`sëk@g
,
wirñëÃWS|ë`së
k
,
sBYy=
pëKkrëqkB67
,
1YsKkrëB67/¨=Å¥X¡;
。
678
:
krì
;
s
;
krâç
9:;<=
:Q939.96 >?@AB:A >CD=:10070435(2015)05103306
犜犺犲犚犲犾犪狋犻狅狀狊犺犻狆犫犲狋狑犲犲狀犗狉犵犪狀犻犮犃犮犻犱犛犲犮狉犲狋犲犱犳狉狅犿犘犺狅狊狆犺狅狉狌狊狊狅犾狌犫犻犾犻狕犻狀犵
犅犪犮狋犲狉犻犪犪狀犱犜犺犲犘犺狅狊狆犺犪狋犲狊狅犾狌犫犻犾犻狕犻狀犵犃犫犻犾犻狋狔
ZHANGYing1,LUGuangxin1,XIEYongli1,YAOTuo2,RONGLiangyan2,ZHUYing2
(1.AgricultureandAnimalHusbandrycolegeofQinhaiUniversity,KeyLaboratoryofAlpineGrasslandEcosysteminthe
SanjiangyuanSourceAreaJointlyFundedbyQinghaiProvinceandMinistryofEducation,Xining,QinhaiProvince810016,China;
2.ColegeofPratacultureScience,GansuAgriculturalUniversity,Lanzhou,GansuProvince730070,China)
犃犫狊狋狉犪犮狋:Inordertoinvestigatethephosphorussolubilizingmechanismofphosphatesolubilizingbacteria,
therelationshipbetweenphosphatesolubilizationabilityandthecategoryoforganicacidproducedbyphos
phatesolubilizingbacteriawasinvestigated.Sixphosphatesolubilizingbacteriascreenedfromrhizosphere
of犕犲犱犻犮犪犵狅狊犪狋犻狏犪,犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊,and犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲aroundLanzhouareawereculturedusing
PKOinorganicphosphorusliquidmedia.Thephosphatesolubilizingcapacityandcategoryoforganicacids
producedbybacteriawerecheckedbymeansofmolybdenumbluecolorimetryandHPLC,respectively.
Theresultsshowedthatthephosphatesolubilizingcapacityofeachbacteriapresentedsignificantdifference
(犘<0.01)andtheincrementofeffectivephosphatewasbetween106.53~373.44mg·L-1.Thecategory
oforganicacidsproducedbyphosphorussolubilizingbacterialincludedoxalicacid,tartaricacid,malic
acid,lacticacid,aceticacid,citricacid,succinicacidandsoon.However,thecategoryandquantityofor
ganicacidssecretedbydifferentstrainshadsignificantdifference.Relativitywasdiscoveredbetweenphos
phatesolubilizingcapacityandtotalorganicacids.Theincrementofeffectivephosphatewasnotentirely
affectedbytotalorganicacid,andphosphatesolubilizingcapacitywasaffectedbycategoryandquantityof
organicacids.Nonetheless,theinfluencedegreeandmechanismoforganicacidseffectedphosphatesolubi
lizingabilitystilneedsfurtherinvestigation.
犓犲狔狑狅狉犱狊:Phosphatesolubilizingbacteria;organicacids;Phosphatesolubilizingability
EFGH
:20141224;IJGH:20150614
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(31260025)(31360584);J32®¯°+±²³(2014ZJ927Q);J3*+ƽ°±²³´µ
OPQR
:
HH
(1977),¸,J3pG»,ƽ,ǽ,¾D¿ÀNñ:=89¼<¢:ìB,Email:zhangying2019@126.
com;ÇÈÅÆ Authorforcorrespondence,Email:yaotuo@gsau.edu.cn
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;%ðìi¿râB:9./ìfkÃ
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r
[7]。
TK6krüì;in\{â,jk
Òkrë=z²sBYypë
,
z{krë
8z²sYy
、
pëB@g
,
GkrXB
ÎXWÔ½
。
1 STaUV
1.1 üFWX
Uìë78()*+N)+0:9=V
ºÎ
,
0#¿ 2
、
H,
、
k3!ñ89¼
(
P1),1ìãöBâRg[813]。
\1 ]^üF
Table1 Testedstrains
ì?
StrainNo.
ì
Resourcesofstrains
â
Function
Hsg 2Lanzhou krPsolubilization
0ÇNfixation
ls213 H,Jiuquan êÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊L.roots
krPsolubilization
z²IAASecretingIAA
ls35 k3 Dingxi êÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊L.roots
krPsolubilization
z²IAASecretingIAA
lhs11 2Lanzhou aÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots
krPsolubilization
z²IAASecretingIAA
lhs6 H,Jiuquan aÙ©t¼ËÌSoilawayfrom犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots
krPsolubilization
z²IAASecretingIAA
lhs8 k3 Dingxi aÙ©t¼ËÌSoilawayfrom犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots krPsolubilization
ò(Note):¡Fk Notidentified
1.2 !:K
B¥LB0\=n\{â;iìBP<=
ìþnBLÕ
[14],
B¥PKOVrn\{â;
iìkrë=z²sYy=pëBjk
[15]。
1.3 ü"D0Ô#
¦4/{â+ûBìLBLh{â
P<
,
ÌzÆAY¦LBn\{â+,¦28℃,
125r·min-1{â48h。¡ìåz:u
Ì
,
|¥VìKRà1ììþnBܨG1×108
cfu·mL-1(~u660nm,ODõ≥0.5)。
1.4 ûm°0fg
150mLÙãi+ël50mLn\PKOV
r{â
,121℃Mì20min,BÉÌ,zƦ500
μL1¡jìBìþn(10
8cfu·mL-1)AY×Ùã
i+
。
ìæ3eCM,}Ã{â(WAYì
)
GK}
。
¦sÙãiZ¦28℃,160r·min-1
4301
!5# H H!:krìz²s8krâç@g
j,{â10d。¦{ân10000r·min-1,4℃
515min,x
(
k$K}ÌBõ
(mg·L-1))[16]。
1.5 ;ýþÿ÷<¥ø°0fg
xs{ân5ÌB
¥ìinÞ
ð
(¨
¬lô Waters475,-sIj Waters
487);isYyÌëjk,Þ:z{
C18,4.6×250mm,Ij~u210nm,èQGpH
=2.75,0.01mol·L-1rsÛËÚ"ín,è¶1.0
mL·min-1,_25℃[17]。
1.6 hijk
p½^XB¥SPSS16.0¿,CÈB¥
LSDÓ。
2 lmY;n
2.1 üFûf$ÿm0*t
}rsÙÙGër
,
*¥ PKOVr
n\{âKUì;in\{â
,
jk{
ânirñë
,
lmPJ
,
1ìkrë*
¦100mg·L-1,ÃDor(犘<0.01)(P
2),irñë106.53~373.44mg·L-1
,
Ò+
,ls213irñë(373.44mg·
L-1)vì,lhs8irñë(367.23mg·L-1)
ü
,Hsg i r ñ ë (106.53mg·L-1)
vÚ
。
\2 üFjkþômÈ°Yþÿ÷<¥h°
Table2 Thenatureandconcentrationoforganicacidsandavailablephosphateincrementindifferenttreatments
^X
Treatments
sYypë
Thenatureandconcentrationoforganicacids/PPM
Ns
Oxalicacid
Hjs
Tartaricacid
dms
Malicacid
ws
Lacticacid
+s
Aceticacid
efs
Citricacid
ñÛs
succinicacid
`së
Totalorganic
acid
irñë
Available
phosphate
increment
/mg·L-1
lhs11 3.33 45.33 115.24 988.11 237.91 - - 1389.88aA 354.82bB
lhs6 12.07 77.43 186.32 70.05 117.99 159.53 - 623.39bB 209.13dD
ls35 8.95 - 48.14 - 361.97 90.62 85.14 594.82bB 283.11cC
ls213 - 56.13 159.52 43.07 132.68 - - 391.40cC 373.44aA
lhs8 53.84 28.18 38.23 - 146.12 - - 266.37dD 367.23aA
Hsg 4.91 56.71 - 24.92 41.59 - - 128.13eE 106.53eE
CK 3.89 10.51 15.37 16.38 32.64 1.45 - 80.24fF 0.00fF
ò:“-”Pó¾Ij;XÄWXwéBCPóDor(犘<0.05);WX*éBCPóDoÃr(犘<0.01)
Note:“-”meansnodetected;Differentsmallettersinthesamecolumnmeansignificantdifferenceat0.05level;Differentcapitalletters
indicatesignificantdifferenceat0.01level
2.2 üF;ýþÿ÷0
s
,
jk1^Xì{ânB`së
、
sB
Yy=pë
(
P2),lmPJ,1ìâz²
s
,
`sëDor
(犘<0.01),lhs11ì
{ân`së
(1389.88PPM)vì,Hsgì
{ân`së
(128.13PPM)vÚ。krì
âz²sBYy¾DNs
、
Hjs
、
dms
、
ws
、
+s
、
efs=ñÛs!
,
1ì{ân
sBYy=pëDo*
,
Clhs11,ls213,lhs8
= Hsgì{ân¾Ijefs=ñÛs,ì
ls35{ân¾IjHjs=ws,ìls35+
sÌë361.97mg·L-18ì Hsg+sÌë
41.59mg·L-1D#10[。Xì{â
n1sBÌëqW&
,
Cìlhs11{ân
wsë988.11mg·L-15Nsë3.33mg·L-1B
300%。T + @ A, i r ñ ë * ¦ 300
mg·L-1B3^X(lhs11,ls213=lhs8){ân
÷sy¾Ij
,
Cefs=ñÛs
,
Ð|â58ù
kgrl@
,
Ñ{ân+BÌëï5
,
½×
,
q
|â5¥¦:9{â/+B1Y?Ñ
,
\ÞEFÅ;
。
2.3 üFûm°Y;ýìþÿ÷°06
^XìPKO{ân+:u10dÌâz
²s=kBkrâç
,
K1ì{ân`
s8irñëB@g;iHz{
(
×1),l
mPJ
,犢
irñë
=0.0917犡
`së
+230.5(犚2=
0.1466),{ânirñë8s`ëfmg
@
(犘<0.05),w@gã。lhs11ì{ân`
së
(1389.88PPM)vì,wirñë(354.82
mg·L-1)Ãmvì,ls213ì{ân`së
5301
! " # $ !23"
(391.40PPM)rÚ¦lhs11ì(犘<0.01),w
irñë
(373.44mg·L-1)Éì¦lhs11ì18.62
mg·L-1。ÒlmPJirñë8s`ë
kB@g
,
wirñëÃWS|ë`
sBë
k
,
#Ò5:9BkrXmnMN
,
5ë¦YEFXÅ¥Blm
。
:1 ûm°Yìþÿ÷°0|63;n
Fig.1 RelationbetweenPsolubiliztionandorganicacids
ò
:犘<0.05,X
Note:犘<0.05,thesameasbelow
2.4 üFûm°Y;ýþÿ÷^XìPKO{ân+:u10dÌ{ân
sBYy=ëWX
,
K1YsBë8
irñëB@g;iHz{
(
×2~7),lmP
J
,
1YsBë8irñëfmg@
(犘
<0.05),w@gã,Ò+,Ns(犢irñë=
1.5277犡
Nsë
+261.22(犚2 =0.0822))、dms
(犢
irñë
= 0.4586犡
dmsë
+ 240.53 (犚2 =
0.1006))、ws(犢
irñë
=0.0854犡
wsë
+266.34
(犚2=0.0989))=+s(犢
irñë
=0.416犡
+së
+
210.4(犚2=0.1898))ë8irñëfð@,
Hjs
(犢
irñë
=-1.3439犡
Hjsë
+341.46(犚2
=0.1145))=efs(犢
irñë
=-0.5001犡
efsë
+303.23(犚2=0.1019))ë8irñëfí
@
。
T+ì]z²BsYyKkrq
kB67
,
efs=ñÛs67GJ
,
WX
s¢;krÅ¥TI@efs
、
ñÛs>Ns、
Hjs
、
dms>+s,wsYyKkrâçB
67/¨øFÅ;
。
3 ~
kr:9âk¿ËÌ+Bùkrsÿ,5
:2 ûm°Y(÷°0|63;n
Fig.2 RelationshipsbetweenPsolubiliztionandoxalicacids
:3 ûm°Y%&÷°0|63;n
Fig.3 RelationshipsbetweenPsolubiliztionandtartaricacids
:4 ûm°Ym÷°0|63;n
Fig.4 RelationshipsbetweenPsolubiliztionandwalicacids
:5 ûm°Y(÷°0|6;n
Fig.5 RelationshipsbetweenPsolubiliztionlacticacids
EGÐ÷:9âz²skrâç
。
è
ð*!
[18]
vG:9z²BsÊÚ°¤
ËÌBpHõ,ÑrsÙ、rsm、rsl!ùk
rsÿsgk¿
,
Xz²Bsâ8
m
、
l
、
Ù
、
Ü!±O7n@]@
,
¦8l@B
6301
!5# H H!:krìz²s8krâç@g
:6 ûm°Y)÷°0|63;n
Fig.6 RelationshipsbetweenPsolubiliztionandaceticacids
:7 ûm°Y*+÷°0|63;n
Fig.7 RelationshipsbetweenPsolubiliztionandcitricacids
rs¼#ò
,
ÄÅ¿rB³B
。
¡}H!
[19]
@
Aüìâ^z²+s
、
)s
、
dms
、
ws
、
efs
!
,
WXìz²BsBYyDoC*
;
:
ìz²BsYy¾Defs
、
Ns
、
+s
、
H
js
、
ws
、
ñÛs!
,
ìz²sYyD
ÆqÈ*
。Cunningham![20],Golstein[21],Kim
!
[22]
= Hameeda![23]@AzQxsq5ËÌ
:9z²BYwz7s+BY
,
Xø
âú:=z²Ò~Ys
,
CNs
、
+s
、
ws
、
Hjs
、
ops
、
efs
,
:9BkrXB
lWq8èð*BlmX
。
qvG:9
BkrL
,
$H8z²s@s
,
ø8z²ù
7
(H+)、#òB CO2 = H2S!YEF@。
Asea![24]j³gËÌ+犘犲狀犻犮犻犾犾犻狌犿犫犻犾犪犼犻=
犘犲狀犻犮犻犾犾犻狌犿犮犳.犳狌狊犮狌犿«ìBkrâç@A,
ONH+4 ì9k¿VrsÿBâ
ç
,
ìBkrëq8pH BÊGö@,}
NH+4 GëÇ,{ânBpH õ|Ê×3.7,
krëqìÄ298mg·L-1;}NO-3 GÇ,
{ânBpHõG6.2,krâçërÊÚ,kr
ëøG7mg·L-1。Ilmer![25]q³Jqì
犘犲狀犻犮犻犾犾犻狌犿犪狌狉犪狀狋犻狅犵狉犻狊犲狌犿 @A,3ìk
¿ÙrsÿBâç
,
wÃWz²s
,
¾D5ë¦
ÏÐHÙ7¿NXrs¼¿Ùrsÿ+¹
。
dÌJq犘犲狀犻犮犻犾犾犻狌犿狊犻犿狆犾犻犮犻狊狊犻犿狌犿
@A
,
|}ÇNH+4 BX<Å¥#òù7,¿Ê
ÚHÜËÌB pH õ,ÎìHrsÿBk¿
¨
[2627]。
qÆvG
,
h*BËÌ:9fÇ´ÏÅ¥#òCO2,kqrCO2 q|N
XËÌpH õBÊÚ,êÅk¿¤zrsÿBÅ
¥
[28];
ËÌ+BÛ3ì|#ò H2S,H2S8FeP<
@9
,
:áÛsÐlÃ#òrs¼
,
vq|
ÄÅk¿¤zùkgVr<@9B³B
。
;BPJ
,
:9z²sú:B
krÅ¥$H8z²sBYy=pë@
s
[29],
ø8rs¼l@à7@
[3031]。Chen
!
[32]
H¿ÂÌ+¤zÅBkrüì
,
¿rÅ
¥8sBú:={âpH õBÊÚ@,;
Ç HPLCz{,ìB{ân+@AHe
fs
、
zQxs
、
ws!8YWXBss,ø@
AH3YWì«Bs。T@Akrìz²
sBYy¾DNs
、
Hjs
、
dms
、
ws
、
+
s
、
efs=ñÛs!
,
irñë8`së
kB@g
,
wirñëÃWS|ë`
së
k
,
sBYy=pëKkrëq
kB67
,
8}ø+ÆBlmX
。
`
,
GHIJkr:9MNBkrL=
krØ
,
ÅÌB|}¿t+
、
z7:9+KL
;ißlB
,
§WXÝÜBkrãä:
9Yf
,
z{1ìBkrR:
,
JµkrìB@q
âERÖL
。
4 l
1ì^Xirñë 106.5~373.4
mg·L-1。krìâz²sBYy¾D
Ns
、
Hjs
、
dms
、
ws
、
+s
、
efs=ñÛ
s!
,
w1ìz²sBYy=pëDo*
。
krë8`sëkB@g
,
wi
rñëÃWS|ë`së
k
,
sBY
y=pëKkrëqkB67
,
67/¨=Å
¥Xø¡;
。
u>?
[1] wr,£¨.:9¿rB;k[J].ËÌ$,2001
(3):711
[2] £¨,wr,ªs/,!._YWX:F+¿rüìB
pëYfzÎ
[J].ËÌ8F,2000,9(1):3437
7301
! " # $ !23"
[3] wr,£¨,ªs/,!.wd¼<8m¼<¿rüìBz
Î
[J].S4(+w,2001,16(1):111115
[4] d±,;:ì,¶(~.¼<¿r:9;k[J].S
9&()*++w
,2006,12(6):4449
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