免费文献传递   相关文献

The Relationship between Organic Acid Secreted from Phosphorus-solubilizing Bacteria and The Phosphate-solubilizing Ability

溶磷菌分泌有机酸与溶磷能力相关性研究



全 文 :!23" !5#
 Vol.23  No.5
! " # $
ACTA AGRESTIA SINICA
   2015$  9%
  Sep.  2015
犱狅犻:10.11733/j.issn.10070435.2015.05.020
ûmü;ýþÿ÷Yûm*t|63”•
) Z1,[>1,\$|1,]  2^,SJ_2, ñ2
(1.J3*+(,+0 Ùn†ÝìæNñ:2¤ƒðC:=Vº,J3 3°810016;
2.78()*+N)+0,78  2730070)
45

GH˜™krìBkr›X

I™krìz²›sBYy=pë8krâçBƒ@g

*¥PKOV›r
n\{â‡K§Œ® 2u¡B(犕犲犱犻犮犪犵狅狊犪狋犻狏犪)、êÙ©(犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊)=aÙ©(犜狉犻犳狅犾犻狌犿狆狉犪狋
犲狀狊犲)¼ë

lmPJ

1ìkrëDo—r
(犘<0.01),irñë•106.53~373.44mg·L-1’“。Uìz²
›sBYy¾DNs

Hjs

dms

ws

+s

efs=ñÛs!

wz²›sBYy=pëDoš*

krìBkrë8`›s듔•Škƒ@g

wirñëÃWS|ë`›së…k

›sBYy=
pëKkrëqŠkB67

1Y›sKkrëB67/¨=Å¥›X¡;ŠŒ˜™

678

krì

›s

krâç
9:;<=
:Q939.96    >?@AB:A     >CD=:10070435(2015)05103306
犜犺犲犚犲犾犪狋犻狅狀狊犺犻狆犫犲狋狑犲犲狀犗狉犵犪狀犻犮犃犮犻犱犛犲犮狉犲狋犲犱犳狉狅犿犘犺狅狊狆犺狅狉狌狊狊狅犾狌犫犻犾犻狕犻狀犵
犅犪犮狋犲狉犻犪犪狀犱犜犺犲犘犺狅狊狆犺犪狋犲狊狅犾狌犫犻犾犻狕犻狀犵犃犫犻犾犻狋狔
ZHANGYing1,LUGuangxin1,XIEYongli1,YAOTuo2,RONGLiangyan2,ZHUYing2
(1.AgricultureandAnimalHusbandrycolegeofQinhaiUniversity,KeyLaboratoryofAlpineGrasslandEcosysteminthe
SanjiangyuanSourceAreaJointlyFundedbyQinghaiProvinceandMinistryofEducation,Xining,QinhaiProvince810016,China;
2.ColegeofPratacultureScience,GansuAgriculturalUniversity,Lanzhou,GansuProvince730070,China)
犃犫狊狋狉犪犮狋:Inordertoinvestigatethephosphorussolubilizingmechanismofphosphatesolubilizingbacteria,
therelationshipbetweenphosphatesolubilizationabilityandthecategoryoforganicacidproducedbyphos
phatesolubilizingbacteriawasinvestigated.Sixphosphatesolubilizingbacteriascreenedfromrhizosphere
of犕犲犱犻犮犪犵狅狊犪狋犻狏犪,犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊,and犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲aroundLanzhouareawereculturedusing
PKOinorganicphosphorusliquidmedia.Thephosphatesolubilizingcapacityandcategoryoforganicacids
producedbybacteriawerecheckedbymeansofmolybdenumbluecolorimetryandHPLC,respectively.
Theresultsshowedthatthephosphatesolubilizingcapacityofeachbacteriapresentedsignificantdifference
(犘<0.01)andtheincrementofeffectivephosphatewasbetween106.53~373.44mg·L-1.Thecategory
oforganicacidsproducedbyphosphorussolubilizingbacterialincludedoxalicacid,tartaricacid,malic
acid,lacticacid,aceticacid,citricacid,succinicacidandsoon.However,thecategoryandquantityofor
ganicacidssecretedbydifferentstrainshadsignificantdifference.Relativitywasdiscoveredbetweenphos
phatesolubilizingcapacityandtotalorganicacids.Theincrementofeffectivephosphatewasnotentirely
affectedbytotalorganicacid,andphosphatesolubilizingcapacitywasaffectedbycategoryandquantityof
organicacids.Nonetheless,theinfluencedegreeandmechanismoforganicacidseffectedphosphatesolubi
lizingabilitystilneedsfurtherinvestigation.
犓犲狔狑狅狉犱狊:Phosphatesolubilizingbacteria;organicacids;Phosphatesolubilizingability
EFGH
:20141224;IJGH:20150614
KLMN

¬­®¯°+‡±²³
(31260025)(31360584);J32®¯°+‡±²³(2014ZJ927Q);J3*+ƽ°˜‡±²³´µ
OPQR

HH
(1977),¸,J3pG»,ƽ,ǖ½,¾D¿ÀNñ:=89¼<¢:ìB˜™,Email:zhangying2019@126.
com;ÇÈÅÆ Authorforcorrespondence,Email:yaotuo@gsau.edu.cn
  r589:ujFBáâ®F’Š,¾D#8
9¼A¿ËÌ+ÏÐ



˜™PJ0¬ËÌB
`rÌëšì

wЇ95%Br[GVir,89
CùÓA*¥

•=<:ú+

»Ó*ë!¥r$…
! " # $ !23"
V×89Búë

w!lBr$*¤zr8ËÌ+
BV›Ÿ7l@

€áHùkgBrsÿ

89KÉ
îr$B*¥5qøG5%~25%[1],89KËÌ
+$%Br†B*¥5mnÚ

hs

•*ë!¥
r$Îì89úëBX“

q&…Hr¯´†B*
ë›y=FŽz…!b‹Ä’

]}

C±ÎìË
ÌùkgrBi<=*¥5½êH./B@ò

ËÌ=Å9¼<”•*ëBœ:9

C‹œ:
9Œ¦ùkr2[23],
¦Ð÷œ
:9À]Gkrì

˒üì

:ì=òmì
),
â¦
V›r<@9z¿B]GV›rœ:9

⦍›
r<@9z¿B]G›rœ:9

+ÆK(
õ



£ñ=Añ!89¼<ËÌB¿rœ:9
pë=Yfl%;iH˜™

@A›rüìYy
ЇV›rüì

›rüì¾DG*g3ìO

Ò
üGVœý3ìO

V›rüì¾DGVœý3ì
O
[2]。
C‹+Æ*¥89¼<œ:9âz¿ùkr
BR:

U×*¥¿rœ:9…P<ËÌ+Bùk
gr

}ÎìrBig

ÐáG1¬+ÆӘ™B
9:’Š
[4]。
#$…

¬‚sB+ÆKkrœ:9BYy=
âç;iH./B˜™
[5],
qxÕHŠkBám

w
‹$B˜™lmqPJ

¿89¼rœ:9

•=Vº˜™‘šPAJ—Bkr
âç

wž¥Å=<:ú+

C‹ìkrimÉÃ
WJ—

ž¥imWX³

˜™ÒÞEvG¾D5ë
¦¿rœ:9YyS‹

¿rœ:9¿r›Xmn
MN

ˆ‰1¿rœ:9B¿r›LqW4ƒX

}øB˜™.ŋ§+¦ìYB§Œ=krâçB
Ț

Kkr›XÃWrzJ‹
[6]。
H¿¿rœ:
9B¿r›L

|}Jµ¿rœ:9B¿r”Ø

G
;ŠŒ%ðìi¿râB:9./ìfk‡Ã

MÒìi¿rBX“qâÊ¿‹YV›r=›
r
[7]。
T˜™K6krüì;in\{â,jk
Òkrë=z²›sBYy†pë

z{krë
8z²›sYy

pë“Bƒ@g

Gkr›XB
˜™ÎXWÔ½

1 STaUV
1.1 üFWX
Uìë78()*+N)+0œ:9=V
ºÎ

0#¿ 2

H,

k3!ñ89¼

P1),1음ãöBâRg[813]。
\1 ]^üF
Table1 Testedstrains
ì?™
StrainNo.
읅†
Resourcesofstrains
â
Function
Hsg  2Lanzhou krPsolubilization
0ÇNfixation
ls213 H,Jiuquan êÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊L.roots
krPsolubilization
z²IAASecretingIAA
ls35 k3 Dingxi êÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狉犲狆犲狀狊L.roots
krPsolubilization
z²IAASecretingIAA
lhs11  2Lanzhou aÙ©¼<ËÌSoiladheringto犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots
krPsolubilization
z²IAASecretingIAA
lhs6 H,Jiuquan aÙ©t¼ËÌSoilawayfrom犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots
krPsolubilization
z²IAASecretingIAA
lhs8 k3 Dingxi aÙ©t¼ËÌSoilawayfrom犜狉犻犳狅犾犻狌犿狆狉犪狋犲狀狊犲L.roots krPsolubilization
  ò(Note):¡Fk Notidentified
1.2 !:K
B¥LB0\=n\{â‡;iìBP<=
ìþnBLÕ
[14],
B¥PKOV›rn\{â‡;
iìkrë=z²›sYy=pëBjk
[15]。
1.3 ü"D0Ô#
¦4/{â‡+û”B읕LBLh{â‡
P<

’ÌzÆAY¦LBn\{â‡+,¦28℃,
125r·min-1‘š{â48h。¡ìåz:u
Ì

|¥VìKRà1ììþnBܨG1×108
cfu·mL-1(~u660nm,ODõ≥0.5)。
1.4 ûm°0fg
•150mLÙãi+ël50mLn\PKOV›
r{â‡
,121℃Mì20min,BɒÌ,zƦ500
μL1¡jìBìþn(10
8cfu·mL-1)AY×Ùã
i+

”ìæ3eCM,}‡Ã{â‡(WAYì


GK}

¦‚sÙãiZ¦28℃,160r·min-1
4301
!5# H H!:krìz²›s8krâçƒ@g˜™
j,{â10d。¦{ân•10000r·min-1,4℃Ÿ
515min,x‚ë

k$K}ÌBõ
(mg·L-1))[16]。
1.5 ;ýþÿ÷„<¥ø°0fg
x‚s{ânŸ5ÌB‚
¥ìinƒÞ
†ð
(¨
¬lô Waters475,-sIj‰ Waters
487);i›sYy†Ìëjk,ކ‘:z{
C18,4.6×250mm,Ij~u210nm,èQƒGpH
=2.75,0.01mol·L-1rsÛËÚ"ín,è¶1.0
mL·min-1,_25℃[17]。
1.6 hijk
p½^XB¥SPSS16.0¿‘,‹CȚB¥
LSDÓ。
2 lmY;n
2.1 üFûf$ÿm0*t
}rsÙÙGëŠr†

*¥ PKOV›r
n\{â‡KUì;in\{â

jk{
ânirñë

lmPJ

1ìkrë–*
¦100mg·L-1,ÉDo—r(犘<0.01)(P
2),irñë•106.53~373.44mg·L-1
’“

Ò+
,ls213irñë(373.44mg·
L-1)vì,lhs8irñë(367.23mg·L-1)
ü ’
,Hsg  i r ñ ë (106.53mg·L-1)


\2 üFjkþômÈ°Yþÿ÷„<¥h°
Table2 Thenatureandconcentrationoforganicacidsandavailablephosphateincrementindifferenttreatments
^X
Treatments
›sYy†pë
Thenatureandconcentrationoforganicacids/PPM
Ns
Oxalicacid
Hjs
Tartaricacid
dms
Malicacid
ws
Lacticacid
+s
Aceticacid
efs
Citricacid
ñÛs
succinicacid
`›së
Totalorganic
acid
irñë
Available
phosphate
increment
/mg·L-1
lhs11 3.33 45.33 115.24 988.11 237.91 - - 1389.88aA 354.82bB
lhs6 12.07 77.43 186.32 70.05 117.99 159.53 - 623.39bB 209.13dD
ls35 8.95 - 48.14 - 361.97 90.62 85.14 594.82bB 283.11cC
ls213 - 56.13 159.52 43.07 132.68 - - 391.40cC 373.44aA
lhs8 53.84 28.18 38.23 - 146.12 - - 266.37dD 367.23aA
Hsg 4.91 56.71 - 24.92 41.59 -  - 128.13eE 106.53eE
CK 3.89 10.51 15.37 16.38 32.64 1.45 - 80.24fF 0.00fF
  ò:“-”Pó¾Ij;XÄWXwéBCPóDo—r(犘<0.05);WX*éBCPóDo×r(犘<0.01)
Note:“-”meansnodetected;Differentsmallettersinthesamecolumnmeansignificantdifferenceat0.05level;Differentcapitalletters
indicatesignificantdifferenceat0.01level
2.2 üF;ýþÿ÷0„C‹œ:9•:¼?ÑPQ+n*z²›
s

jk1^Xì{ânB`›së

›sB
Yy=pë

P2),lmPJ,1읖âz²›
s

‰`›sëDo—r
(犘<0.01),lhs11ì
{ân`›së
(1389.88PPM)vì,Hsgì
{ân`›së
(128.13PPM)vÚ。krì
âz²›sBYy¾DNs

Hjs

dms

ws

+s

efs=ñÛs!

1읓{ân
›sBYy=pëDoš*

Clhs11,ls213,lhs8
= Hsgì{ân¾Ijefs=ñÛs,ì
ls35{ân¾IjHjs=ws,ìls35+
sÌë361.97mg·L-18ì Hsg+sÌë
41.59mg·L-1ƒD#10[。ˆ‰XŠì{â
n1›sBÌëqWŠ&

Cìlhs11{ân
wsë988.11mg·L-15Nsë3.33mg·L-1B
300%。T ˜ ™ + @ A, i r ñ ë * ¦ 300
mg·L-1B3^X(lhs11,ls213=lhs8){ân
÷sy¾Ij

Cefs=ñÛs

Ð|â58ù
kgrl@

Ñ{ân+BÌëï5

½×›“

q
|â5¥¦œ:9{â‡/+B1Y?ѝž

Œ
\ÞEFÅ;ŠŒ˜™

2.3 üFûm°Y;ýìþÿ÷°06‚
^X읕PKO{ân+:u10d̖âz
²›s=ŒŠkBkrâç

K1ì{ân`
›s8irñëBƒ@g;iHz{

×1),l
mPJ
,犢
irñë
=0.0917犡
`›së
+230.5(犚2=
0.1466),{ânirñë8›s`ë“fmg
ƒ@
(犘<0.05),wƒ@gšã。lhs11ì{ân`
›së
(1389.88PPM)vì,wirñë(354.82
mg·L-1)Ãmvì,ls213ì{ân`›së
5301
! " # $ !23"
(391.40PPM)—rÚ¦lhs11ì(犘<0.01),w
irñë
(373.44mg·L-1)Éì¦lhs11ì18.62
mg·L-1。ÒlmPJirñë8›s`ë“
”•ŠkBƒ@g

wirñëÃWS|ë`›
sBë…k

#Ò5œ:9Bkr›XmnMN

5릋YEFƒXÅ¥Blm

:1 ûm°Yìþÿ÷°0|63;n
Fig.1 RelationbetweenPsolubiliztionandorganicacids
ò
:犘<0.05,šX
Note:犘<0.05,thesameasbelow
2.4 üFûm°Y;ýþÿ÷„^X읕PKO{ân+:u10dÌ{ân
›sBYy=ë–WƒX

K1Y›sBë8
irñëBƒ@g;iHz{

×2~7),lmP
J

1Y›sBë8irñë“fmgƒ@
(犘
<0.05),wƒ@g–šã,Ò+,Ns(犢irñë=
1.5277犡
Nsë
+261.22(犚2 =0.0822))、dms
(犢
irñë
= 0.4586犡
dmsë
+ 240.53 (犚2 =
0.1006))、ws(犢
irñë
=0.0854犡
wsë
+266.34
(犚2=0.0989))=+s(犢
irñë
=0.416犡
+së

210.4(犚2=0.1898))ë8irñë“fðƒ@,
ˆHjs
(犢
irñë
=-1.3439犡
Hjsë
+341.46(犚2
=0.1145))=efs(犢
irñë
=-0.5001犡
efsë
+303.23(犚2=0.1019))ë8irñë“fíƒ
@

T˜™+ì]z²B›sYyKkrq
ŠkB67

efs=ñÛs67šGJ—

WX
›s¢;krÅ¥‡TI@efs

ñÛs>Ns、
Hjs

dms>+s,w›sYyKkrâçB
67/¨øFÅ;ŠŒ˜™

3 ~
  krœ:9âk¿ËÌ+Bùkrsÿ,5
:2 ûm°Y(÷°0|63;n
Fig.2 RelationshipsbetweenPsolubiliztionandoxalicacids
:3 ûm°Y%&÷°0|63;n
Fig.3 RelationshipsbetweenPsolubiliztionandtartaricacids
:4 ûm°Ym÷°0|63;n
Fig.4 RelationshipsbetweenPsolubiliztionandwalicacids
:5 ûm°Y(÷°0|6;n
Fig.5 RelationshipsbetweenPsolubiliztionlacticacids
EGÐ÷œ:9âz²›sˆŒkrâç

è
ð*!
[18]
˜™vGœ:9z²B›sÊÚ°¤
ËÌBpHõ,ÑrsÙ、rsm、rsl!ùk
rsÿ•sg‘šk¿

X“z²B›sâ8
m

l

Ù

Ü!±OŸ7n@]”@

¦8’l@B
6301
!5# H H!:krìz²›s8krâçƒ@g˜™
:6 ûm°Y)÷°0|63;n
Fig.6 RelationshipsbetweenPsolubiliztionandaceticacids
:7 ûm°Y*+÷°0|63;n
Fig.7 RelationshipsbetweenPsolubiliztionandcitricacids
rs¼#ò…

ÄÅ¿rB³B

¡}H!
[19]
@
Aüìâ^z²+s

)s

dms

ws

efs
!

WX읒“z²B›sBYyDoC*

:
ìz²B›sYy¾Defs

Ns

+s

H
js

ws

ñÛs!

읒“z²›sYyD
ÆqȚ*
。Cunningham![20],Golstein[21],Kim
!
[22]
= Hameeda![23]˜™@AzQxsq5ËÌ
œ:9z²B‹Ywz7›s+BŠY

X“ø
âú:=z²Ò~‹Y›s

CNs

+s

ws

Hjs

ops

efs

˜™œ:9Bkr›XB
lWq8èð*BlmŠX

q˜™vGœ:9
Bkr›L

$H8z²›s@s

ø8z²ù
7
(H+)、#òB CO2 = H2S!‹YEFƒ@。
Asea![24]˜™j³gËÌ+犘犲狀犻犮犻犾犾犻狌犿犫犻犾犪犼犻=
犘犲狀犻犮犻犾犾犻狌犿犮犳.犳狌狊犮狌犿«ìBkrâç“@A,
ONH+4 ”•“ì9Œk¿V›rsÿBâ
ç

ìBkrëq8pH BšÊGöƒ@,}
NH+4 GëŠÇ†“,{ânBpH õ|Ê×3.7,
krëqìÄ298mg·L-1;ˆ}NO-3 Gdž“,
{ânBpHõG6.2,krâçë—rÊÚ,kr
ëøG7mg·L-1。Ilmer![25]˜™q³Jqì
犘犲狀犻犮犻犾犾犻狌犿犪狌狉犪狀狋犻狅犵狉犻狊犲狌犿 @A,3음k
¿ÙrsÿBâç

wÃWz²›s

¾D5ë¦
ÏÐHٟ7¿ˆNXrs¼¿Ùrsÿ+¹Ÿ
…

d̘™”Jq犘犲狀犻犮犻犾犾犻狌犿狊犻犿狆犾犻犮犻狊狊犻犿狌犿
@A

|}LJNH+4 BX<Å¥#òù7,¿ˆÊ
ÚHœÜËÌB pH õ,ÎìHrsÿBk¿
¨
[2627]。
q˜™ÆvG

h*BË̜:9fLJ´ÏÅ¥#òCO2,Škqr‚CO2 q|N
XËÌpH õBÊÚ,êÅk¿¤zrsÿBÅ
¥
[28];
ËÌ+BÛ3ì|#ò H2S,H2S8FeP<
@9ž

:áÛsÐlÃ#òrs¼

vžq|
ÄÅk¿¤zùkgV›r<@9B³B

;ŠŒB˜™PJ

œ:9z²›sú:B
krÅ¥$H8z²›sBYy=pë@
s
[29],
ø8rs¼l@àŸ7@
[3031]。Chen
!
[32]
˜™H¿ÂÌ+¤zŸÅBkrüì

¿rÅ
¥8›sBú:={â‡pH õBÊڍ@,;
ŠŒÇ‡ HPLCz{,•ìB{ân+@AHe
fs

zQxs

ws!8YWXB›ss,ø@
AH3YWì«B›s。T˜™@Akrìz²
›sBYy¾DNs

Hjs

dms

ws

+
s

efs=ñÛs!

irñë8`›së“
”•ŠkBƒ@g

wirñëÃWS|ë`
›së…k

›sBYy=pëKkrëq
ŠkB67

8}ø+ÆB˜™lmƒŠX



GHIJkrœ:9MNBkr›L=
krӯ

ÅÌB˜™|}¿t+

z7:9+KL
‚;ißlB˜™

§ŒWXÝÜBkrãäœ:
9Yf

z{1ìBkrR:

JµkrìB@q
â‡E†R֛L

4 l
1ì^Xirñë• 106.5~373.4
mg·L-1’“。krìâz²›sBYy¾D
Ns

Hjs

dms

ws

+s

efs=ñÛ
s!

w1ìz²›sBYy=pëDoš*

krë8`›s듔•ŠkBƒ@g

wi
rñëÃWS|ë`›së…k

›sBY
y=pëKkrëqŠkB67

67/¨=Å
¥›Xø¡;ŠŒ˜™

u>?
[1] ’wr,£‹¨.œ:9¿rB˜™;k[J].ËÌ$‘,2001
(3):711
[2] £‹¨,’wr,ªs/,!._YWX:FŽ+¿rüìB
pë†YfzÎ
[J].ËÌ8FŽ,2000,9(1):3437
7301
! " # $ !23"
[3] ’wr,£‹¨,ªs/,!.wd¼<8m¼<¿rüìBz
Î
[J].S4(+w,2001,16(1):111115
[4] šd±,;:ì,¶(~.¼<¿rœ:9˜™;k[J].S
9&()*++w
,2006,12(6):4449
[5] ¶Ô,õ¡~,²aØ,!.Ë̜:9krz7›X˜™;k
[J].ËÌ+w,2009,46(5):925931
[6] GyaneshwarP,NareshKG,ParekhLJ,犲狋犪犾.Roleofsoil
microorganismsinimprovingPnutritionofplants[J].Plant
andSoil,2002,245(1):8393
[7] ;t~,¦·ì,f@X,!.œ:9¿r›XB˜™;k[J].
ܬ()°+
,2008(2):8891
[8] ֐k,^ u,$­,!. 2ñ݌†ì¤zRg˜™
[J].+¬Nñ+w,2009,31(1):45
50
[9] $­,^ u,֐k,!.aÙ©¼‹I
[J].Nñ+w,2009,17(2):259263
[10]$­.Ù©N¼8()*+¼½+YW˜
,2009:2528
[11]HH,$­,^ u.Ù©N¼k
[J].NÞ8NŒ,2012,32(5):3640
[12]HH,$­,^ u,!.zŸ®,N¼<_¢:ì(PGPR)
JÅiž˜™
[J].N)+w,2013,22(1):2937
[13]HH,^ u,$­.M@AYÊKÙ©N:uRg=Gù67
˜™
[J].89áâ8$‘+w,2012,18(5):12771285
[14]Mgœ,^ u,u¢3,!.krì=0Çìk¿r¯š“BJ
Åiž
[J].:+w,2006,26(8):27642769
[15]HafeezFY,MalikKA.ManualonBiofertilizerTechnology
[M].Pakistan:NIBGE,2000
[16]SmithKP,GoodmanR M.Hostvariationforinteractions
withbeneficialplantassociatedmicrobes[J].AnnualReview
Phytopathology,1999,37:473491
[17]t+î,;Ÿç,vJ,,!.ûo¼<¿rìB¿râç=z
²›sB‹Œjk
[J].œ:9+NZ,2007,27(5):7074
[18]èð*,j›W.rüì$‘B˜™=ž¥AhÌs[J].<$
.)
,2005(4):1820
[19]¡}H,±&„,nžW.ìi¿rìK¯9ÄgÏ­rB2
<†:9igB67
[J].on*++w:()8:¼°+
¡
,2000,26(4):461464
[20]CunninghamJE,KuiackC.Productionofcitricandoxalic
acidsandsolubilizationofcalciumphosphatesby犘犲狀犻犮犻犾犾犻狌犿
犫犻犾犪犻犻[J].AppliedandEnvironmentalMicrobiology,1992,58
(5):14511458
[21]GolsteinA H.Bacterialphosphatesolubilization Historical
perspectiveandfutureprospects[J].AmAltAgric,1986(1):
5157
[22]KimKY,JordanD,McDonaldGA.Effectofphosphatesol
ubilizingbacteriaandvesiculararbuscularmycorrhizaeonto
matogrowandsoilmicrobialactivity[J].BiologyandFertility
ofSoils,1997,26(2):7987
[23]HameedaB,ReddyYH,RupelaOP,犲狋犪犾.Effectofcarbon
substratesonrockphosphatesolubilizationbybacteriafrom
compostsandmacrofauna[J].CurrentMicrobiology,2006,53
(4):298302
[24]AseaPEA,KuccyRMN,StewartJWB.Inorganicphos
phatesoluilizationbytwopeniciliumspeciesinsolutionculture
andsoil[J].SoilBiologyBiochemstry,1988,20(4):459464
[25]IlmerP,SchinnerF.Solubilizaofinorganiccalciumphos
phaterssolubilization mechanisms[J].Soil Biology Bio
chemstry,1995,27(3):257263
[26]IlhnerP,BarbatoA,SchinnerF.Solubilizationofhardlysol
ubleAlPO4withPsoluilizingmicroorganisms[J].SoilBiology
Biochemstry,1995,27(3):265270
[27]AhujaA,GhoshSB,D’SouzaSF.Isolationofastarchutili
zingphosphatesolubilizingfungusonbufferedmediumandits
characterization[J].BioresourceTechnology,2007,98(17):
34083411
[28]GhaniA,RajanSSS,LeeA.Enhancementofphosphate
rocksolubilitythroughbiologicalprocesses[J].SoilBiology
Biochemstry,1994,26(1):127136
[29]LouwH A,WebleyD M.Astudyofsoilbacteriadissolving
certain mineralphospnatefertilizersandrelatedcompounds
[J].AppliedBacteriology,1959,22(3):227233
[30]PiciniD,AzconR.Effectofphosphatesolubilzingbacteriaand
vesiculararbuscularmycorrhizalfungiontheutilizationof
Bayovarrockphosphatebyalfalfaplantsusingasandvermic
ulitemedium[J].PlantandSoil,1987,101(1):4550
[31]Zë,Ô:,֍õ,!.WXKz=® 9‘Kj³gË
ÌV›r€2[J].89áâ8$‘+w,2002,8
(1):5864
[32]ChenYP,RekhaPD,ArunAB,犲狋犪犾.Phosphatesolubilizing
bacteriafromsubtropicalsoilandtheirtricalciumphosphate
solubilizingabilities[J].AppliedSoilEcology,2006,34(1):33
41

%&( )*+)
8301