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Identification of Pathogen Causing Phoma Leaf Spot on Medicago sativa in Shaanxi Province

陕西省苜蓿茎点霉叶斑病病原菌的鉴定



全 文 :!23" !6#
 Vol.23  No.6
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犐犱犲狀狋犻犳犻犮犪狋犻狅狀狅犳犘犪狋犺狅犵犲狀犆犪狌狊犻狀犵犘犺狅犿犪犔犲犪犳犛狆狅狋狅狀
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LIHongjian1,LIFanghong1,SHIJian2,HuTianming1,
ChengJiming1,QIANYonghua1,JIAOFeng1
(1.ColegeofAnimalScienceandTechnology,NorthWestAgricultureandForestryUniversity,Yangling,Shaanxi
Province712100,China;2.AnimalhealthinspectionofXipocountry,Binxian,ShaanxiProvince713500,China)
犃犫狊狋狉犪犮狋:犘犺狅犿犪Leafspotisaseriousleafdiseaseinalfalfa,whichhashappenedinShaanxiProvincein
recently.Inordertofindoutthepathogenofthisdiseaseexactly,thediseasedleaftypicalsymptomwas
thatsomesepiaspotstogetherexpandlikea"annulation"scab,andtheconidiawerehyaline,cylindrical,
amerosporeandunicelular,andthesizewas7×1.25μm.Thephylogenetictreeofthepathogennucleo
tidesequenceofinternaltranscribedspacer(ITS)shared99%similaritywiththatof犘犺狅犿犪犿犲犱犻犮犪犵犻狀犻狊
狏犪狉.犕犲犱犻犮犪犵犻狀犻狊(DQ109960)inNeighbourJoining(NJ)tree,or98%similarityusingunweightedpair
groupmethodinarithmeticmeans(UPGMA)tree.Theinoculationofexcisedleaftissueverifiedthatthe
pathogenhadpathogenicity.Theresultsindicatedthatitwascausedby犘犺狅犿犪犿犲犱犻犮犪犵犻狀犻狊狏犪狉.犕犲犱犻
犮犪犵犻狀犻狊.
犓犲狔狑狅狉犱狊:犕犲犱犻犮犪犵狅狊犪狋犻狏犪;Pathogen;rDNAITS;犘犺狅犿犪Leafspot;Excisedleaftissueinoculation
CDEF
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Number
IdentI
Identvalue
犘犺狅犿犪犿犲犱犻犮犪犵犻狀犻狊 MP22 KF181254.1 99%
犘犺狅犿犪犿犲犱犻犮犪犵犻狀犻狊狏犪狉. P3 DQ109960.1 99%
犘犺狅犿犪犺犲狉犫犻犮狅犾犪 CBS629.97 GU237898.1 98%
犇犻犱狔犿犲犾犾犪狆犺犪犮犪犲 CBS184.55 EU167570.1 98%
犘犺狅犿犪犿犪犮狉狅狊狋狅犿犪 DAOM DQ474091.1 98%
犃狊犮狅犮犺狔狋犪狉犪犫犻犲犻 CBS237.37 EU167600.1 98%
犃狊犮狅犮犺狔狋犪狆犻狊犻狏犪狉.狆犻狊犻 CBS108.26 EU167557.1 97%
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犕犻犮狉狅狊狆犺犪犲狉狅狆狊犻狊狅犾犻狏犪犮犲犪 CBS303.68 JX681101.1 97%
犕犻犮狉狅狊狆犺犪犲狉狅狆狊犻狊狆狉狅狋犲犪犲 CPC1423 JN712495.1 97%
犘犺狅犿犪犮狉狔狊狋犪犾犾犻犳犲狉犪 CanS65 JF817335.1 97%
犇狅狋犺犻狅狉犲犾犾犪犵狉犲犵犪狉犻犪 CanS66 JF817336.1 97%
犃狊犮狅犮犺狔狋犪狏犻犮犻犪犲狏犻犾犾狅狊犪犲 CBS255.92 EU167560.1 97%
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›[[1] ä³Õ,€>%,È*=.=>UV[M]./V:=>4ªSC
K
,1995:114130
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[3] €@,?E,æ\%,¡.|Óÿœ`¿çY¶žYq{Go
[J].{),2009,28(6):765768
[4] HÊ,ì¨Ú,ž­,¡.犘犺狅犿犪e犘犺狔犾犾狅狊狋犻犮狋犪 e!x1lD
E»«
[J].\,4ª()),1995,17(4):102107
[5] AkamatsuH O,ChilversMI,PeeverTL.Firstreportof
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犻犮犳狌狀犵犻from犔犻狏犻狊狋狅狀犪犮犺犻狀犲狀狊犻狊basedonmorphologyand
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