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作 者 ：彭小伟;柳俊;李运广;罗玮;蔡明红;何剑锋

期 刊 ：武汉植物学研究 2010年 28卷 1期 页码：56-62

关键词：UV-B辐射；光合色素；比生长率；SOD；MDA；

Keywords:UV-B radiation, Photosynthesis pigments, Relative growth, SOD, MDA,

摘 要 ：

为了了解微藻对UV-B辐射增强效应的响应,以一种分离纯化于北极冰川融水的淡水微藻(Chlorellasp.)为实验材料,在不同强度UV-B辐射下对其生长、生化组分和细胞超微结构等进行了研究.研究结果显示:3种不同强度的UV-B(22 μW/cm~2,45 μW/cm~2,70 μW/cm~2)辐射均可导致藻的比生长速率及色素含量下降,且辐射强度越强,两者的下降越明显;而MDA含量和SOD活性会随辐照强度的增强而提高.表明辐射强度增强,UV-B对藻的伤害程度加大,而该小球藻SOD活性随UV-B强度增强而提高,表明其对上升的UV-B辐射有一定的适应能力.

Abstract:The response of a microalgae(Chlorella sp.)strain which isolation and purification from Arctic glacial meltwater to the enhancement of UV-B radiation was explored in this study. The effects of three different UV-B radiation, i. e., 22 μW/cm~2, 45 μW/cm~2, 70 μW/cm~2 were tested on the algal growth, biochemical components, and cell ultrastructure. The results showed that UV-B irradiation could inhibit the relative growth and reduce content of pigment;and the in-hibiting effects were more significant according to the intensity increasing. Meanwhile, the SOD activity and MDA contents of the strain increased with higher UV-B irradiation. The results showed that the enhancement of UV irradiation could deepen the damage of Chlorella species and algae could adapt the higher intensity of UV-B radiation to some extent.

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