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作 者 ：程强强;庄东红;许大熊;李军;杨志娟;杜虹

期 刊 ：植物科学学报 2011年 29卷 4期 页码：524-530

关键词：蝴蝶兰；叶片培养；噻重氮苯基脲；不定芽；基因型；植株再生；

Keywords:Phalaenopsis, Leaf culture, Thidiazuron, Adventitious shoots, Genotypes, Plant regeneration,

摘 要 ：以蝴蝶兰(Phalaenopsis)无菌幼苗叶片为材料,研究添加TDZ(噻重氮苯基脲)条件下不同基因型、激素组合、叶片大小、暗培养时间对不定芽发生和再生植株的影响。结果表明:在相同培养条件下,不同基因型外植体芽诱导率差异显著,‘红天使’最高,达81.5%,‘汕农姑娘’等2个品种为0,‘满天红’等4个品种为9.2%～34.9%;添加TDZ芽诱导率显著高于6-BA;单独添加TDZ或6-BA芽诱导率显著高于NAA与TDZ或6-BA的组合。叶片越小不定芽诱导率越高;短时间暗处理有利于不定芽的发生。以1～2 cm长叶片为材料、15 d暗处理、在1/2 MS添加3 mg/L TDZ培养基中,‘红天使’的叶片外植体芽诱导率和平均不定芽数分别可达100.0%和18.2个。研究发现,在继代培养中TDZ对芽的伸长有抑制作用。

Abstract:The effects of different genotypes,plant growth regulators(PGRs) combination,leaf size and dark treatment on adventitious shoot formation and plant regeneration from leaf explants of Phalaenopsis with thidiazuron(TDZ) added were studied.The difference in the induction rate of adventitious shoots among different genotypes with same induction conditions was significant,for example,the ’Red Angle’ was the highest,up to 81.5%,Doritaenopsis Shannong ’Lassie’ and one other were 0,Doritaenopsis Queen Beer ’Red Sky’ and two others were up to 9.2%-34.9%.The adventitious shoots induction rate under TDZ treatment was significantly greater than that in the 6-BA with the same genotype.The adventitious shoots induction rate with 6-BA or TDZ added alone was superior to the combination of 6-BA or TDZ with NAA.When the leaves were younger,the adventitious shoots induction rate was higher.Short dark treatment was propitious to highly significant on adventitious shoot formation.Using 1-2 cm leaves as material,with 15 days of dark treatment and the 3 mg/L TDZ treatment in 1/2 MS culture medium,the explants induction rate and average number of adventitious shoots of ’Red Angel’ reached 100% and 18.2,respectively.This study showed that bud elongation was obviously inhibited by TDZ treatment in subculture medium.

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