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作 者 ：段艳欣;郭文武

期 刊 ：武汉植物学研究 2010年 28卷 1期 页码：105-108

关键词：总多酚；Folin-Ciocalteu(福林酚)法；柑橘胚性愈伤组织；基因型；

Keywords:Total polyphenols, Folin-Ciocalteu, Citrus embryogenic callus, Genotype,

摘 要 ：研究了甲醇水溶液浓度和浸提时间、品种/基因型、培养时间对柑橘胚性愈伤组织总多酚含量的影响。结果表明,用40%的甲醇溶液在55℃水浴条件下浸提30min,柑橘胚性愈伤组织总多酚的得率最高;总多酚含量受品种/基因型的影响,在所比较的6个柑橘品种/基因型暗柳橙、椪柑、默科特橘橙、无酸甜橙、锦橙和红马叙葡萄柚中,暗柳橙胚性愈伤组织的总多酚含量最高;总多酚含量随培养时间的改变而变化,在培养40d时各品种/基因型胚性愈伤组织的总多酚含量均达到最大值。

Abstract:The influences of methanol/water solution, extracting time, cultivar/genotype, and culturing days on extraction of total polyphenols in citrus embryogenic calli were studied. The results showed that the highest production of total polyphenols was obtained when citrus calli were homogenized with 40% methanol and incubated at 55℃ for 30 min. Total polyphenol content was cultivar/genotype dependent and highest in ’Anliucheng’ sweet orange compared with that in ’ Jincheng’, ’ Succari’ sweet orange, ’ Ponkan’ tangerine, ’ Murcutt’ hybrid, and ’ Red Marsh’ grapefruit. In addition, total polyphenol content was changed with culturing days, and the highest production of total polyphenols was obtained in citrus calli 40 d after culturing.

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