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作 者 ：廖思红, 陈建军, 王瑛

期 刊 ：植物科学学报 2013年 31卷 4期 页码：385-390

关键词：偏斜淫羊藿；类黄酮；超声提取；LC-MS；

Keywords:Epimedium truncatum, Flavonoid, Ultrasonic assisted extraction, LC-MS,

摘 要 ：采用70%乙醇超声波辅助提取偏斜淫羊藿(Epimedium truncatum H.R.Liang)叶片中的类黄酮化学成分,利用LC-MS联用技术鉴定其化合物组成,利用HPLC方法测定其主要活性成分的含量并与药典规定药材箭叶淫羊藿(Epimedium sagittatum)主要活性成分的含量进行比较。结果从偏斜淫羊藿叶片中鉴定出6个化合物:朝藿定B、朝藿定C、淫羊藿苷、3,5,7-三羟基4‘-甲氧基-8-异戊烯基黄酮-3-O-α-L-吡喃鼠李糖基(1→2)-α-L-吡喃鼠李糖苷、槲皮素-3-O-鼠李糖甙、山奈苷。该6个化合物均为首次在该植物中发现。偏斜淫羊藿富含朝藿定C,其含量显著高于箭叶淫羊藿主要活性成分的总含量。结果表明LC-MS可以简单、快速地对淫羊藿化学成分进行定性和定量分析,偏斜淫羊藿富含朝藿定C,具有潜在的药用价值。

Abstract:Flavonoids of Epimedium truncatum were extracted by ultrasonic assisted extraction with 70% ethanol.The extract was analyzed by capillary liquid chromatography-mass spectrometry,and the main active compounds were determined by HPLC and compared with that of Epimedium sagittatum.Six constituents,including epimedin B,epimedin C,icariin,quercetin-3-O-rhamnoside,kaempferin and 3,5,7-Trihydroxyl-4‘-methoxyl-8-prenylflavone-3-O-α-L-rhamno-pyranosyl-(1→2)-α-L-rhamnopyranoside,were isolated and identified from leaves of E.truncatum for the first time.Leaves of E.truncatum contained high levels of epimedin C,which exceeded that of E.sagittatum significantly.Results showed that LC-MS was a quick and easy method for compound analysis of herba epimedii.E.truncatum is a good plant resource for epimedin C and worth further exploration.

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