Abstract:The ability of morphogenesis in anther culture of Hyoscyamus niger was quite different when anthers were cultured in Bourgin and Nitsch‘s(BN)medium supplemented with fifteen gradient concentrations(0.01=100mg/L)of β-naphthoxyacetic acid(NOA). Three kinds of developmental pathway were observed: 1. pollen grain gave rise to embryoids directly; 2. pollen grain initiated callus from which embryoids developed and 3. buds and roots were produced from pollen callus.It was cvident from the results that the pattern of development was similar to cither embryoids derived from pollen grain directly or from callus. The embryoids were differentiated through typical globular-shaped, heartshaped; torpedo-shaped and cotyledonary stages similar to those of zygotic embryos. The differences between two kinds of embryogenesis are the origin of proembryoid.The initiation of bud and root from pollen callus are discussed.