虽然已经有大豆下胚轴再生植株的很多报道，但再生率总是很低并且不定芽的再生都是在添加细胞分裂素的培养基上取得的。这种培养基使外植体形成大量愈伤组织且阻碍了再生芽的进一步生长。为此本实验将表面灭菌的成熟种子置2种培养基上萌发，一种添加2 mg/L苄基腺嘌呤（BA）而另一种不添加；6 d后将小苗的下胚轴切下作为外植体培养到两种诱导不定芽再生的培养基上，同样一种添加2 mg/L BA而另一种不添加。在9个当地、2个美国和1个巴西共12个供试品系中，最好的培养结果是在含2 mg/L BA的培养基上萌发、长 苗、切取下胚轴并在不含BA的再生培养基诱导不定芽。以此程序，不定芽的再生率和质量都得到了显著提高，其中一个当地品种“广大粒”的再生率达到了72%。
Plant regeneration from soybean hypocotyl explants has already been reported many times but the regeneration rates were always low, and in all the cases adventitious buds were obtained on media containing cytokinin, which causes the production of excessive amount of callus on the explants and hinder the further growth of the regenerated buds. To tackle these problems, experiments were designed where surface-sterilized mature seeds of soybean were placed on two kinds of germination media, one contained 2 mg/L benzyl adenine (BA) but the other not, and hypocotyl explants were obtained from six days old seedlings and inoculated on two regeneration media, one contained 2 mg/L BA but the other not, for the induction of adventitious bud formation. With all the 12 soybean cultivars tested including 9 local, 2 from USA and 1 Brazilian, the best results were all obtained from the treatment using hypocolyl explants from seedlings germinated on medium containing BA and cultured on the BA-free regeneration medium without any exception. By this treating method, the regeneration rate was significantly increased with the highest of 72% from a local cultivar Guangdali, and the quality of the regenerated buds was also improved.
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AmarasingheA A Y等：无细胞分裂素培养基上高效的大豆下胚轴外植体器官再生 图版Ⅰ
Amarasinghe A A Y et al.: High Efficient Organogenic Regeneration from Hypocotyl Explants of Soybean
(Glycine max L.) on BA-free Medium PlateⅠ
A: Culture responses of hypocotyl explants in different medium combinations
B: Rooting of regenerated shoots in medium C: Growth and development of regenerated
shoots in a pod
A1 A2
A3 A4
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